Mechanism of Crassostrea gigas T-box Transcription Factor 2: Regulation by a Transcript Isoform of Microphthalmia-Associated Transcription Factor and Its Role in Cell Proliferation

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wenhao Yang, Jinhai Zhang, Yvlu Jiang, Chen Liu, Yijing Han, Yaqiong Liu, Xiaotong Wang
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引用次数: 0

Abstract

T-box transcription factor 2 (TBX2) plays a critical role in various biological processes, including cell cycle regulation, malignant transformation, and regulating melanogenesis. In our previous study, we identified a Crassostrea gigas TBX2 (CgTBX2) and investigated its regulatory role in melanin production in oysters. Here, the mechanism of CgTBX2 in regulating cell proliferation was investigated. First, we found that CgTBX2 promoted the proliferation of mouse melanoma (B16F10) cells. CgMITF-X3, a 1347 bp transcript isoform of MITF from C. gigas, was then cloned and it was also found to promote cell proliferation. Co-transfection of CgTBX2 and CgMITF-X3 into B16F10 cells had a synergistic effect on cell proliferation, suggesting that CgMITF-X3 enhanced the function of CgTBX2 in promoting cell proliferation. CgMITF-X3 promoted the transcriptional activation of CgTBX2 by directly binding to the TCTCACGCGG sequence in the CgTBX2 promoter region. In addition, CgTBX2 and CgMITF-X3 proteins were co-located in the nucleus, indicating that these two proteins may perform a certain function collectively in the nucleus. Taken together, our findings revealed that CgTBX2 is directly activated by CgMITF-X3 at the transcriptional level, and both CgTBX2 and CgMITF-X3 facilitate cell proliferation.

长牡蛎T-box转录因子2的机制:小眼相关转录因子的转录异构体调控及其在细胞增殖中的作用
T-box转录因子2 (T-box transcription factor 2, TBX2)在细胞周期调控、恶性转化、黑色素形成等多种生物学过程中发挥着重要作用。在我们之前的研究中,我们鉴定了一种长牡蛎TBX2 (CgTBX2),并研究了它在牡蛎黑色素生成中的调节作用。本研究探讨了CgTBX2调控细胞增殖的机制。首先,我们发现CgTBX2促进了小鼠黑色素瘤(B16F10)细胞的增殖。CgMITF-X3是C. gigas MITF的一个1347 bp的转录异构体,随后被克隆出来,并且发现它也能促进细胞增殖。CgTBX2和CgMITF-X3共转染B16F10细胞,对细胞增殖具有协同作用,说明CgMITF-X3增强了CgTBX2促进细胞增殖的功能。CgMITF-X3通过直接结合CgTBX2启动子区域的TCTCACGCGG序列,促进CgTBX2的转录激活。此外,CgTBX2和CgMITF-X3蛋白在细胞核中共定位,表明这两种蛋白可能在细胞核中共同发挥一定的功能。综上所述,我们的研究结果表明CgTBX2在转录水平上直接被CgMITF-X3激活,CgTBX2和CgMITF-X3都促进细胞增殖。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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