Preparation and identification of a novel antioxidative peptide from fermented protein hydrolysate of chicken (Gallus gallus domesticus) meat

Abstract

Present investigation deals with the preparation, purification, and identification of antioxidant peptides from fermented chicken (Gallus gallus domesticus) meat protein hydrolysate (CMPH). Antioxidant CMPH was produced by Pediococcus pentosaceus fermentation using 45 % chicken meat concentration, 2 % dextrose, and 48 h of fermentation time. Antioxidant peptides were separated from CMPH by employing ultrafiltration (3 kDa MWCO), Sephadex G-15 gel filtration chromatography, and RP-HPLC, respectively. Ultrafiltration (3 kDa MWCO) revealed that CMPH-UF-1 (MW < 3 kDa) exhibited significantly higher ferric-reducing antioxidant power (FRAP) (22.70 µM TE/mg), DPPH (30.77 µM TE/mg), and ABTS radical scavenging activity (39.41 µM TE/mg) and Fe²⁺ chelating activity (21.45 µM EDTA/mg) than CMPH-UF-2 (p < 0.05). Six fractions of antioxidant peptides were separated from CMPH-UF-1 by Sephadex G-15 chromatography. Among these, CMPH-GF-5 peptide fraction unveiled significantly higher antioxidant activities with FRAP (38.21 µM TE/mg), DPPH radical scavenging activity (55.91 µM TE/mg), and ABTS radical scavenging activity (68.41 µM TE/mg) and Fe²⁺ chelating activity (36.96 µM EDTA/mg) (p < 0.05). Subsequent RP-HPLC purification of CMPH-GF-5 produced eleven fractions, among which CMPH-RPH-8 showed superior antioxidant activities with FRAP (65.06 µM TE/mg), DPPH radical scavenging activity (110.2 µM TE/mg), and ABTS radical scavenging activity (94.07 µM TE/mg) and Fe²⁺ chelating activity (56.19 µM EDTA/mg). LC-MS/MS analysis identified a novel peptide in CMPH-RPH-8 (857.898 Da) with the sequence Leu-Gly-Gln-Glu-Ser-Leu-Ser-Pro-Asp, which has not been previously reported. Identified peptide was synthesized and verified the antioxidant activities. Therefore, the purified antioxidant peptide from CMPH is novel and can serve as a promising antioxidant in functional foods and nutraceutical products.