Tissue distribution, excretion characteristics and metabolic profiling studies of oxypeucedanin in rats using liquid chromatography tandem mass spectrometry

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-02-19 DOI:10.1016/j.jchromb.2025.124525

Huixiao Duo , Liyun Wang , Pei Lu , Xiaodan Zhang , Mingcong Zheng , Hanying Song , Juan Zhou , Wenzhuo Lei , Shushu Ding , Jie Jia Li , Junxu Li , Qing Zhu

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引用次数: 0

Abstract

Oxypeucedanin (OPD) is a linear furanocoumarin extracted from Chinese herbs, which has been reported to exhibit various pharmacological effects such as analgesia, antitussive, anticancer and anti-inflammatory activities. In this paper, the tissue distribution, excretion and metabolism characteristics of OPD in rat was conducted in order to better understand this compound and for potential development of OPD as a medicine. We first used the established HPLC/MS/MS method to examine the plasma protein binding rate and the tissue distribution of OPD in rats after oral administration. The excretion characteristics of OPD in urine, feces and bile, as well as its metabolic profiles in plasma, urine and bile were also investigated. The results demonstrated that after OPD administration, extensive metabolism occurred in rats, involving first-pass effect and enterohepatic circulation, with significant sex differences observed in the metabolic process. The study identified eight metabolites in rats, indicating that OPD metabolism primarily occurs through oxidation, reduction, and glucuronidation. In summary, this study systematically examined the basic process of OPD in vivo, providing a robust foundation for accurately predicting the behavior, efficacy and safety of OPD in humans.

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用液相色谱串联质谱法研究氧去核苷在大鼠体内的组织分布、排泄特征及代谢谱

氧去核苷（OPD）是一种从中草药中提取的线性呋喃香豆素，具有镇痛、止咳、抗癌、抗炎等药理作用。为了更好地了解OPD在大鼠体内的组织分布、排泄和代谢特征，以及OPD作为药物的开发潜力，本文对OPD在大鼠体内的组织分布、排泄和代谢特征进行了研究。我们首先采用建立的HPLC/MS/MS方法检测口服给药大鼠血浆蛋白结合率和OPD的组织分布。研究了OPD在尿液、粪便和胆汁中的排泄特征，以及其在血浆、尿液和胆汁中的代谢谱。结果表明，OPD给药后，大鼠发生了广泛的代谢，涉及首过效应和肠肝循环，代谢过程存在显著的性别差异。该研究确定了大鼠体内的8种代谢物，表明OPD代谢主要通过氧化、还原和葡萄糖醛酸化发生。综上所述，本研究系统地考察了OPD在体内的基本过程，为准确预测OPD在人体内的行为、疗效和安全性提供了坚实的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.