Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b

IF 4.5 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

New biotechnology Pub Date : 2025-02-19 DOI:10.1016/j.nbt.2025.02.004

Diep Thi Ngoc Nguyen , Ok Kyung Lee , Jeong-Geol Na , Eun Yeol Lee

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引用次数: 0

Abstract

We report a biosynthetic method for producing poly(3-hydroxybutyrate-co-3-hydroxypropionate) [P(3HB-co-3HP)] copolymer from methane alone; we developed a 3-hydroxypropionate (3HP) biosynthetic pathway starting from methane in Methylosinus trichosporium OB3b and overexpressed 3HP-CoA transferase to optimize 3HP incorporation into P(3HB-co-3HP) copolymers. Upon comparing the β-alanine and malonyl-CoA pathways, we discovered that the latter showed greater potential for the formation of 3HP monomers. In addition, we examined the activities of 3HP-CoA synthetases from three distinct sources and found that the enzyme from Metallosphaera sedula was the most effective for 3HP incorporation. Using methane as the sole carbon source in a shake-flask culture, we developed recombinant strains and found that the best strain was OB3b-MCRM-3S, which formed a P(3HB-co-3HP) copolymer, up to 25.62 % of the biomass, with a maximum 3HP level of 9.02 mol%. Our research demonstrates the successful production of a biopolymer, the P(3HB-co-3HP) copolymer, via methane bioconversion using methanotrophs.

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来源期刊

New biotechnology 生物-生化研究方法

CiteScore

11.40

自引率

1.90%

发文量

审稿时长

1 months

期刊介绍： New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.