The co-occurrence of tet(X4) and tmexCD2-toprJ2 mediated tigecycline resistance in Raoultella ornithinolytica

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2025-02-19 DOI:10.1016/j.jgar.2025.01.021

Weishuai Zhai , Lu Liu , Jijun Kang , Mengjin Xiao , Yiqing Wang , Yao Wang , Yingbo Shen , Congming Wu , Jianzhong Shen , Yang Wang , Dejun Liu

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Abstract

Objectives

This study aimed to characterize the co-occurrence of the tigecycline resistance determinants tet(X4) and tmexCD2-toprJ2 in a Raoultella ornithinolytica isolate collected from a pig rectal swab at the slaughterhouse.

Methods

The R. ornithinolytica isolate WS60 was subjected to antimicrobial susceptibility testing. Whole-genome sequencing (WGS) was performed to analyze the genetic features of the plasmids carrying tet(X4) and tmexCD2-toprJ2. Additionally, a conjugation assay was conducted to evaluate the transferability of these plasmids, followed by a 15-day stability test to assess the persistence of the two resistance determinants.

Results

R. ornithinolytica WS60 exhibited high-level tigecycline resistance, with a minimum inhibitory concentration (MIC) of 32 μg/mL, and was also resistant to ampicillin, ampicillin-sulbactam, chloramphenicol, tetracycline, sulfamethoxazole-trimethoprim, florfenicol, and streptomycin. WGS analysis revealed that WS60 harbored three plasmids, including a 384,249-bp tmexCD2-toprJ2-carrying IncQ plasmid (pWS60–1) and a 78,159-bp tet(X4)-carrying IncFII plasmid (pWS60–2). Interestingly, pWS60–2 was identical to several plasmids found in Klebsiella spp. isolated from animals, animal-derived food, and humans. Moreover, pWS60–2 was successfully transferred to Klebsiella spp. via conjugation, whereas pWS60–1 failed to transfer. Notably, no significant fitness cost was observed in the transconjugants carrying pWS60–2. Additionally, a 15-day stability assay demonstrated that both resistance determinants were stably maintained in the bacterial population without significant loss, underscoring their persistence over time.

Conclusions

This is the first report of the co-occurrence of tet(X4) and tmexCD2-toprJ2 in R. ornithinolytica. Enhanced surveillance in slaughterhouses, along with targeted interventions, should be implemented to mitigate the potential spread of mobile tigecycline resistance throughout the food production chain.

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溶鸟拉乌尔菌中tet（X4）和tmexCD2-toprJ2共同介导的替加环素耐药

目的：本研究旨在描述从屠宰场采集的猪直肠拭子中分离的溶禽拉oultella分离株中泰加环素耐药决定因子tet（X4）和tmexCD2-toprJ2的共存特征。方法：采用抗菌药敏试验对分离株WS60进行检测。采用全基因组测序（WGS）分析携带tet（X4）和tmexCD2-toprJ2的质粒的遗传特征。此外，进行了偶联试验以评估这些质粒的可转移性，随后进行了15天的稳定性试验以评估两个抗性决定因素的持久性。结果：ornithinolytica菌株WS60对替加环素具有较高的耐药性，最低抑制浓度（MIC）为32 μg/mL，对氨苄西林、氨苄西林-舒巴坦、氯霉素、四环素、磺胺甲恶唑-甲氧苄啶、氟苯尼考、链霉素也有耐药。WGS分析显示WS60含有3个质粒，包括384,249 bp的携带tmexd2 - toprj2的IncQ质粒（pWS60-1）和78,159 bp的携带tet（X4）的IncFII质粒（pWS60-2）。有趣的是，pWS60-2与从动物、动物源性食物和人类分离的克雷伯氏菌中发现的几个质粒相同。此外，pWS60-2成功地通过接合转移到克雷伯氏菌，而pWS60-1未能转移。值得注意的是，在携带pWS60-2的异共轭子中没有观察到显著的适应度成本。此外，一项为期15天的稳定性试验表明，这两种耐药决定因素在细菌种群中都能稳定维持，没有明显的损失，强调了它们随时间的持久性。结论：这是首次报道tet（X4）和tmexCD2-toprJ2在溶鸟鼠中同时存在。应加强屠宰场的监测，同时实施有针对性的干预措施，以减轻流动替加环素耐药性在整个食品生产链中的潜在传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.