Unveiling the Antibacterial, Antifungal, and Cytotoxic Potential of Selenium Nanoparticles Synthesized with Syzygium cumini Leaf Extract

IF 2.7 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR

Journal of Cluster Science Pub Date : 2025-02-22 DOI:10.1007/s10876-025-02777-3

Haorongbam Joldy Devi, Prathiba Gnanasekaran, Arumugam Madan Kumar, Durairaj Siva, Yumnam Asha Devi

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引用次数: 0

Abstract

The study aimed to utilize ecofriendly synthesis to produce selenium nanoparticles using Syzygium cumini leaf extract (SC-SeNPs), and assessing their antibacterial, antifungal activity, and cytotoxic effects. The synthesized Sc-SeNPs were initially subjected to various characterization studies to explore its properties. UV-Visible (UV-Vis) spectroscopy exhibited maximum absorption at 255 nm, while Fourier Transform Infra-Red (FTIR) spectroscopy confirmed synthesis by identifying functional groups. X-ray diffractometer (XRD) analysis indicated a nanocrystalline structure, and Field Emission Scanning Electron Microscopy (FESEM) imaging showed spherical SC-SeNPs with an average size of 32 ± 11 nm. Energy-Dispersive X-ray (EDX) spectroscopy confirmed Selenium (Se) presence in the nanoparticles (NPs). The SC-SeNPs exhibited antibacterial effectiveness against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Klebsiella pneumoniae NTCC 13439, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ranging from 50 to 400 µg/ml and 100–800 µg/ml, respectively. Additionally, SC-SeNPs exhibited antifungal activity against Candida albicans ATCC 10231, Mucor sp. ATCC 52912 and Aspergillus niger ATCC 16888, with MIC and minimum fungicidal concentration (MFC) ranging from 3.125 to 25 µg/ml and 3.125–50 µg/ml, respectively, displaying bactericidal and fungicidal effects against pathogens. In cytotoxicity test, there was significant reduction (p˂0.05) in cell viability compared to the control group after the concentration of 250 µg/ml. It showed low toxic effect with IC₅₀ value of 295 µg/ml on Vero cell lines. The findings suggest SC-SeNPs’ potential as antibacterial and antifungal agents.

Graphical Abstract

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揭示用芫荽叶提取物合成的硒纳米颗粒的抗菌、抗真菌和细胞毒潜能

本研究旨在利用生态合成技术制备纳米硒，并评估其抗菌、抗真菌活性和细胞毒作用。合成的Sc-SeNPs最初进行了各种表征研究以探索其性质。紫外-可见（UV-Vis）光谱在255 nm处显示出最大吸收，而傅里叶变换红外（FTIR）光谱通过鉴定官能团证实了合成。x射线衍射（XRD）分析表明SC-SeNPs为纳米晶结构，场发射扫描电镜（FESEM）成像显示SC-SeNPs为球形，平均尺寸为32±11 nm。能量色散x射线（EDX）光谱证实了纳米粒子（NPs）中硒（Se）的存在。SC-SeNPs对金黄色葡萄球菌ATCC 29213、铜绿假单胞菌ATCC 27853、大肠杆菌ATCC 25922和肺炎克雷伯菌NTCC 13439的抑菌效果较好，最小抑菌浓度（MIC）为50 ~ 400µg/ml，最小杀菌浓度（MBC）为100 ~ 800µg/ml。此外，SC-SeNPs对白色念珠菌ATCC 10231、毛霉ATCC 52912和黑曲霉ATCC 16888均表现出抗真菌活性，MIC和最小杀真菌浓度（MFC）分别为3.125 ~ 25µg/ml和3.125 ~ 50µg/ml，显示出对病原菌的杀菌和杀真菌作用。在细胞毒性试验中，浓度为250µg/ml后，细胞活力较对照组显著降低（p小于0.05）。对Vero细胞株具有低毒作用，IC50值为295µg/ml。研究结果表明SC-SeNPs具有抗菌和抗真菌的潜力。图形抽象

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来源期刊

Journal of Cluster Science 化学-无机化学与核化学

CiteScore

6.70

自引率

0.00%

发文量

166

审稿时长

3 months

期刊介绍： The journal publishes the following types of papers: (a) original and important research; (b) authoritative comprehensive reviews or short overviews of topics of current interest; (c) brief but urgent communications on new significant research; and (d) commentaries intended to foster the exchange of innovative or provocative ideas, and to encourage dialogue, amongst researchers working in different cluster disciplines.