Spermatogenesis and ultrastructure of the spermatozoa at three ploidy levels in the bivalve species, Corbicula fluminea

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-02-17 DOI:10.1016/j.tice.2025.102797

Jie Pi , Yangxin Tang , Ying Fu , Xu Wang , Linwei Liu , Xinhua Liu , Jianguo Xiang , Deliang Li

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引用次数: 0

Abstract

A histological and ultrastructural examination of spermatogenesis and spermatozoa in diploid, triploid and tetraploid Corbicula fluminea was conducted. In the Yangtze River estuary, the spermatozoa of C. flumiena exhibited a primitive uniflagellate structure, whereas a modified biflagellate morphology was observed in inland freshwater habitats. This study presents the initial observation of sperm morula in spermatogenesis in C. fluminea. The mature spermatozoa were released as single cells and as spermatozeugmata. It was observed that uniflagellate spermatozoa undergo two meiotic divisions during it formation, whereas biflagellate spermatozoa undergo only one. The uniflagellate and biflagellate spermatozoa were observed to comprise of three distinct parts: the head, middle piece and tail. The longest and shortest spermatozoa (69.24 μm ± 1.70 vs 54.11 μm ± 1.26) were both biflagellate spermatozoa, originating from tetraploid and diploid individuals, respectively. The number of mitochondria was observed to increase in accordance with ploidy. In the uniflagellate spermatozoa, the two centrioles were observed to be oriented perpendicular to each other. In contrast, the two centrioles were oriented in parallel with each other in biflagellate spermatozoa, with each centriole attaching a flagellum. The undulating membrane of the tail contains a substantial number of glycogen granules. The differences in spermiogenesis, sperm morphology and structure between C. flumiena from estuarine (amphogenesis and in vitro development) and inland freshwater habitats (androgenesis and brooding larvae in the inner demibranch) result in better adaptations to the respective reproduction modes.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.