The acute and sub-chronic toxicological effects of 3-amino-9-ethylcarbazole (AEC) on zebrafish.

Erna Vásárhelyi, Gergely Rácz, Béla Urbányi, Balázs P Szabó, Dóra Szepesi-Bencsik, István Szabó, Illés Bock, Cintia Volner, Jeffrey Daniel Griffitts, Balázs Kriszt, Katalin Bakos, Zsolt Csenki
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Abstract

Introduction: In this study, we sought to determine the sub-chronic toxicological effects of AEC on zebrafish embryos.

Methods: We utilized fish early life stage (FELS) and fish embryo toxicity (FET) tests, vascular, neurological, and renal transgenic zebrafish lines, and gene expression anal-ysis of the zebrafish tissue.

Results: In the FET tests, AEC caused several abnormalities in the larvae, with the LC50 at 24 hpf being 4.076 ± 0.221 mg/L and 3.296 ± 0.127 mg/L at 96 hpf. In the FELS test, AEC was shown to be lethal following 16 days of exposure at 0.5 mg/L, 1 mg/L and 2 mg/L. Some of the transgenic zebrafish lines exhibited slight changes in fluorescent signaling pat-terns after exposure to AEC at 1 mg/L and 2 mg/L. Notable results of the gene expression analysis revealed: gpx4b and got2 were downregulated in the liver; HIF1a was downregulated at 0.25 mg/L and 0.5 mg/L concentrations, NOTCH1a and fli-1 genes were downregulated at all concentrations, and A2b was upregulated in the vasculature; a1T, ngn1, elavl3, syn2a, mbp, gap43 were down-regulated in the nervous system; and wt1b was downregulated in the kidney.

Discuccion: Altogether, the results of our study indicate the potential for AEC to cause harm to organisms.

3-amino-9-ethylcarbazole (AEC) 对斑马鱼的急性和亚慢性毒理效应。
简介:本研究试图确定 AEC 对斑马鱼胚胎的亚慢性毒性影响:在这项研究中,我们试图确定 AEC 对斑马鱼胚胎的亚慢性毒性影响:方法:我们利用鱼类早期生命阶段(FELS)和鱼类胚胎毒性(FET)试验、血管、神经和肾脏转基因斑马鱼品系以及斑马鱼组织的基因表达分析:在 FET 试验中,AEC 会导致幼鱼出现多种异常,24 hpf 时的半数致死浓度为 4.076 ± 0.221 mg/L,96 hpf 时的半数致死浓度为 3.296 ± 0.127 mg/L。在 FELS 试验中,浓度为 0.5 毫克/升、1 毫克/升和 2 毫克/升的 AEC 在接触 16 天后会致死。一些转基因斑马鱼品系在接触 1 毫克/升和 2 毫克/升浓度的 AEC 后,荧光信号转导模式发生了轻微变化。基因表达分析的显著结果显示:gpx4b 和 got2 在肝脏中下调;HIF1a 在 0.25 mg/L 和 0.5毫克/升浓度下下调,NOTCH1a和fli-1基因在所有浓度下均下调,A2b在血管中上调;a1T、gn1、elavl3、syn2a、mbp、gap43在神经系统中下调;wt1b在肾脏中下调:总之,我们的研究结果表明 AEC 有可能对生物体造成危害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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