{"title":"Alcohol dehydrogenase iron containing 1 (<i>ADHFE1</i>) is associated with liver metastasis and regulates cellular function of gastric cancer.","authors":"Ling Qin, Fei Liu, Chen Sun, Zhang Zhang, Lingling Sun, Xingli Tang, Yunhong Xia","doi":"10.1177/03936155251319784","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Liver metastasis and chemotherapy resistance are two unfavorable factors for the outcomes of gastric cancer patients. <i>ADHFE1</i> was identified as cancer-related molecule and candidate for early detection in gastric cancer. This study evaluated the significance of <i>ADHFE1</i> in gastric cancer, aiming to explore a potential biomarker and therapeutic target for gastric cancer.</p><p><strong>Methods: </strong>The expression of <i>ADHFE1</i> was detected by polymerase chain reaction and compared between 143 gastric cancer patients and 54 individuals with benign lesions. The role of <i>ADHFE1</i> in gastric cancer development and prognosis was assessed. The effect of <i>ADHFE1</i> on gastric cancer cell growth and motility was evaluated by cell counting kit-8 and Transwell assay. The angiogenesis and cisplatin resistance were assessed by related markers and the half maximal inhibitory concentration values.</p><p><strong>Results: </strong>Significant upregulation of <i>ADHFE1</i> was observed in gastric cancer patients compared to individuals with benign lesions, which showed close association with the tumor node metastasis stage, lymph node metastasis, liver metastasis, and adverse prognosis of gastric cancer patients. Patients with liver metastasis showed higher serum <i>ADHFE1</i> levels, which decreased after chemotherapy. Silencing <i>ADHFE1</i> significantly suppressed gastric cancer cell proliferation, migration, invasion, and angiogenesis while its overexpression showed opposite effects. Moreover, the overexpression of <i>ADHFE1</i> significantly improved the cisplatin resistance of gastric cancer cells.</p><p><strong>Conclusion: </strong>Serum <i>ADHFE1</i> served as an indicator for poor prognosis and liver metastasis of gastric cancer. <i>ADHFE1</i> regulates gastric cancer cell progression, cisplatin resistance, and angiogenesis, suggesting its potential to serve as a therapeutic target.</p>","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"3936155251319784"},"PeriodicalIF":2.3000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Markers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/03936155251319784","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Liver metastasis and chemotherapy resistance are two unfavorable factors for the outcomes of gastric cancer patients. ADHFE1 was identified as cancer-related molecule and candidate for early detection in gastric cancer. This study evaluated the significance of ADHFE1 in gastric cancer, aiming to explore a potential biomarker and therapeutic target for gastric cancer.
Methods: The expression of ADHFE1 was detected by polymerase chain reaction and compared between 143 gastric cancer patients and 54 individuals with benign lesions. The role of ADHFE1 in gastric cancer development and prognosis was assessed. The effect of ADHFE1 on gastric cancer cell growth and motility was evaluated by cell counting kit-8 and Transwell assay. The angiogenesis and cisplatin resistance were assessed by related markers and the half maximal inhibitory concentration values.
Results: Significant upregulation of ADHFE1 was observed in gastric cancer patients compared to individuals with benign lesions, which showed close association with the tumor node metastasis stage, lymph node metastasis, liver metastasis, and adverse prognosis of gastric cancer patients. Patients with liver metastasis showed higher serum ADHFE1 levels, which decreased after chemotherapy. Silencing ADHFE1 significantly suppressed gastric cancer cell proliferation, migration, invasion, and angiogenesis while its overexpression showed opposite effects. Moreover, the overexpression of ADHFE1 significantly improved the cisplatin resistance of gastric cancer cells.
Conclusion: Serum ADHFE1 served as an indicator for poor prognosis and liver metastasis of gastric cancer. ADHFE1 regulates gastric cancer cell progression, cisplatin resistance, and angiogenesis, suggesting its potential to serve as a therapeutic target.
期刊介绍：
IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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