Overexpression of inflammatory human caspase-4 in relation to clinical severity of oral lichen planus.

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2025-02-10 DOI:10.1016/j.oooo.2025.02.003

Darussakorn Maneerat, Anupong Jeerachaipansakul, Chanipa Atijit, Chavanya Tangjarroenphakdee, Panatda Tipsirisakun, Nattapat Hengsanankul, Warisara Krisanaprakornkit, Suttichai Krisanaprakornkit, Anupong Makeudom

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引用次数: 0

Abstract

Objective: Involvement of non-canonical inflammasome, comprising inflammatory human caspase-4, caspase-5, and Gasdermin D, in the pathogenesis of oral lichen planus (OLP) has never been demonstrated. We aimed to determine human caspase-4, caspase-5, and Gasdermin D expressions in OLP, to correlate their expressions with OLP severity, and to measure salivary interleukin (IL)-1β levels.

Study design: OLP and normal oral mucosal specimens (n = 42 each) were processed for immunohistochemistry or immunoblotting. The clinical score for OLP severity was assessed at the most severe site. The immunohistochemical (IHC) score was a summation of intensity and positive cell scores. Salivary IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA).

Results: Median IHC scores of caspase-4 and Gasdermin D in OLP group were significantly greater than those in normal mucosal group (P < .01), consistent with significantly upregulated expressions by immunoblotting (P < .05). The IHC scores of caspase-4 and Gasdermin D were positively correlated with the clinical scores (P < .05). Salivary IL-1β levels in the OLP group were significantly greater than those in the normal mucosal group (P < .001).

Conclusions: Our study demonstrates enhanced human caspase-4 and Gasdermin D expressions in relation to increased OLP severity with elevated salivary IL-1β levels, proposing clinical applications of these biomolecules as potential prognostic markers and/or new therapeutic intervention for OLP.

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.