Isolation and Characterization of Getah Virus GD2202 from Mosquitoes in Foshan, China.

IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES
Vector borne and zoonotic diseases Pub Date : 2025-04-01 Epub Date: 2025-02-21 DOI:10.1089/vbz.2024.0100
Ying-Ling Deng, Zi-Yi Lin, Rui Lin, Zi-Shuo Lu, Xing-Xiu Yan, Li-Xia Li, Hui-Jun Lu, Xue Bai, Ning-Yi Jin, Hao Liu
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Abstract

Background: Getah virus (GETV) is a vector-borne virus that can proliferate in mosquitoes and be transmitted to host animals through bites. Clinical infection with the virus mainly causes diarrhea and reproductive disorders in pigs and fever, rash, and edema in horses. This caused huge losses to the pig industry and also affected the level of competition for horses. GETV has proliferated across over 20 regions within China, recently resulting in miscarriages and fatalities among the pig population, Guangdong province. Currently, there are no highly effective preventative or therapeutic strategies for diseases induced by GETV. Understanding the infection pathways, inclusive of GETV transmission vectors, is of paramount importance for the prevention and management of the disease. Results: To clarify the main transmission vectors and genotypes of GETV in this area, 3600 mosquitoes of different species were collected and GETV was detected using quantitative reverse transcription PCR. The minimum infection rate was 1.36 for Culex tritaeniorhynchus and 0.83 for Anopheles sinensis. The GETV GD2202 strain was successfully isolated from C. tritaeniorhynchus using mouse neuroblastoma (N2a) cells, and its complete genome was sequenced by PCR. This represents the first identification and isolation of GETV from mosquitoes in Guangdong Province of Southern China. Comparison with GenBank data showed 99.3% identity with the E2 gene of the GDFS2-2018 and GDFS9-2018 strains causing disease in local pig populations, and a nucleotide similarity of 99.8% with the E2 gene of the HNNY-1, HNPDS-1, and HNPDS-2 strains isolated from pigs in Henan, and the highest identity with the JL1708 strain isolated from mosquitoes in Jilin Province, with a nucleotide similarity in the E2 gene of 99.9%. Phylogenetic analysis showed that it had the closest genetic evolutionary relationship with the Culex-derived JL1708 strain and was on the same evolutionary branch as the pig-derived HNNY-1, HNPDS-1, and HNPDS-2 strains. Conclusion: A comprehensive investigation was undertaken to examine the prevalence of GETV infection among various mosquito species in Foshan, Guangdong Province, China. The findings indicated that C. tritaeniorhynchus acted as the principal vector for transmission, predominantly infected with GETV genotype III. This genotype was consistent with that identified in deceased pig populations and demonstrated significant homology. This study provides a robust scientific basis for understanding the propagation of GETV, thereby offering vital insights for the formulation of disease prevention and control strategies.

佛山蚊源Getah病毒GD2202的分离与鉴定
背景:Getah病毒(GETV)是一种媒介传播的病毒,可在蚊子体内增殖并通过叮咬传播给宿主动物。临床感染该病毒主要导致猪腹泻和生殖障碍,马发烧、皮疹和水肿。这给养猪业造成了巨大损失,也影响了马匹的竞争水平。GETV已在中国20多个地区扩散,最近导致广东省猪群流产和死亡。目前,对于GETV引起的疾病还没有非常有效的预防或治疗策略。了解感染途径,包括GETV传播媒介,对疾病的预防和管理至关重要。结果:为明确该地区GETV的主要传播媒介和基因型,采集不同种类蚊子3600只,采用定量反转录PCR检测GETV。三带喙库蚊和中华按蚊的最低感染率分别为1.36和0.83。利用小鼠神经母细胞瘤(N2a)细胞从三带喙库蚊中成功分离到GETV GD2202菌株,并对其全基因组进行了PCR测序。这是中国南方广东省首次从蚊虫中分离到GETV。与GenBank数据比较显示,该病毒与本地猪发病株GDFS2-2018和GDFS9-2018的E2基因同源性为99.3%,与河南猪分离株HNNY-1、HNPDS-1和HNPDS-2的E2基因同源性为99.8%,与吉林省蚊源分离株JL1708的E2基因同源性最高,同源性为99.9%。系统发育分析表明,该毒株与库克斯衍生的JL1708毒株的遗传进化关系最为密切,与猪衍生的HNNY-1、HNPDS-1和HNPDS-2毒株在同一进化分支上。结论:对广东省佛山市各蚊种GETV感染情况进行了全面调查。结果表明,三带喙库蚊是主要传播媒介,以感染GETV基因型为主。该基因型与在死亡猪群中鉴定的基因型一致,并表现出显著的同源性。该研究为了解GETV的传播提供了坚实的科学基础,从而为制定疾病预防和控制策略提供了重要的见解。
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来源期刊
CiteScore
4.70
自引率
4.80%
发文量
73
审稿时长
3-8 weeks
期刊介绍: Vector-Borne and Zoonotic Diseases is an authoritative, peer-reviewed journal providing basic and applied research on diseases transmitted to humans by invertebrate vectors or non-human vertebrates. The Journal examines geographic, seasonal, and other risk factors that influence the transmission, diagnosis, management, and prevention of this group of infectious diseases, and identifies global trends that have the potential to result in major epidemics. Vector-Borne and Zoonotic Diseases coverage includes: -Ecology -Entomology -Epidemiology -Infectious diseases -Microbiology -Parasitology -Pathology -Public health -Tropical medicine -Wildlife biology -Bacterial, rickettsial, viral, and parasitic zoonoses
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