{"title":"Fine-mapping of <i>PmHHM</i>, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew.","authors":"Bisheng Fu, Zhixin Lin, Lijuan Yan, Qiaofeng Zhang, Caiyun Liu, Jin Cai, Wei Guo, Ying Liu, Wenling Zhai, Shuangjun Gong, Feng Xu, Jizhong Wu","doi":"10.3389/fpls.2024.1489013","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Common wheat is a leading global food crop that impacts food security. Wheat powdery mildew (PM), caused by <i>Blumeria graminis</i> f. sp. <i>tritici</i> (<i>Bgt</i>), poses a significant threat to grain yield and flour quality. The identification and utilization of broad-spectrum resistance genes against PM are essential for effective disease control.</p><p><strong>Methods: </strong>The resistance spectrum test during the seedling stage and the identification of resistance during the adult stage were conducted to evaluate the wheat landrace Honghuamai (HHM). Five segregating populations were investigated to assess the inheritance of PM resistance in HHM. To map its PM resitance gene, bulked segregant analysis, molecular mapping and comparative genomic analysis were also used in the present study.</p><p><strong>Results: </strong>HHM shows remarkable adult resistance in the field and is nearly immune to all 25 <i>Bgt</i> isolates used in seedling tests, making it an excellent source of PM resistance. PM resistance in HHM was determined by a single dominant gene, temporarily named <i>PmHHM</i>. It was then fine-mapped to an interval with a genetic distance of 0.0031 cM and a physical distance of 187.4 kb on chromosome 4AL of the Chinese Spring reference sequence v.2.1. Four genes were identified in the target region, three of which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative genomic analysis revealed presence/absence variations (PAVs) of the <i>PmHHM</i> locus among common wheat varieties.</p><p><strong>Discussion: </strong>These closely linked molecular markers will not only benefit the cloning of the gene underlying <i>PmHHM</i> but also facilitate the efficient utilization of the gene in breeding programs.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"15 ","pages":"1489013"},"PeriodicalIF":4.1000,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839664/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2024.1489013","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Common wheat is a leading global food crop that impacts food security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses a significant threat to grain yield and flour quality. The identification and utilization of broad-spectrum resistance genes against PM are essential for effective disease control.
Methods: The resistance spectrum test during the seedling stage and the identification of resistance during the adult stage were conducted to evaluate the wheat landrace Honghuamai (HHM). Five segregating populations were investigated to assess the inheritance of PM resistance in HHM. To map its PM resitance gene, bulked segregant analysis, molecular mapping and comparative genomic analysis were also used in the present study.
Results: HHM shows remarkable adult resistance in the field and is nearly immune to all 25 Bgt isolates used in seedling tests, making it an excellent source of PM resistance. PM resistance in HHM was determined by a single dominant gene, temporarily named PmHHM. It was then fine-mapped to an interval with a genetic distance of 0.0031 cM and a physical distance of 187.4 kb on chromosome 4AL of the Chinese Spring reference sequence v.2.1. Four genes were identified in the target region, three of which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative genomic analysis revealed presence/absence variations (PAVs) of the PmHHM locus among common wheat varieties.
Discussion: These closely linked molecular markers will not only benefit the cloning of the gene underlying PmHHM but also facilitate the efficient utilization of the gene in breeding programs.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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