Acute oxygen sensing by arterial chemoreceptors with a mutant mitochondrial complex I ND6 subunit lacking reverse electron transport

IF 3.5 4区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
María Torres-López, Pedro F. Spiller, Lin Gao, Paula García-Flores, Michael P. Murphy, Patricia Ortega-Sáenz, José López-Barneo
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引用次数: 0

Abstract

Carotid body glomus cells are essential for stimulating breathing in response to hypoxia. They contain specialized mitochondria in which hypoxia induces the accumulation of NADH and H2O2 that modulate membrane ion channel activity. We investigated whether hypoxia induces reverse electron transport (RET) at mitochondrial complex I (MCI). We studied glomus cells from mice with a mutation in ND6, a core protein of MCI, which maintain normal MCI NADH dehydrogenase activity but cannot catalyze RET. The ND6 mutation increases the propensity of MCI to deactivate, and glomus cells with deactivated MCI are insensitive to acute hypoxia. These findings further indicate that MCI function is necessary for glomus cell responsiveness to hypoxia, although MCI RET does not seem to be required for this process.

Abstract Image

具有线粒体复合物I ND6亚基突变体的动脉化学受体缺乏反向电子传递的急性氧感知。
颈动脉体血管球细胞对缺氧时刺激呼吸至关重要。它们含有特殊的线粒体,其中缺氧诱导NADH和H2O2的积累,从而调节膜离子通道的活性。我们研究了缺氧是否会诱导线粒体复合体I (MCI)的反向电子传递(RET)。我们研究了MCI核心蛋白ND6突变小鼠的球囊细胞,ND6突变可以维持正常的MCI NADH脱氢酶活性,但不能催化RET。ND6突变增加了MCI失活的倾向,MCI失活的球囊细胞对急性缺氧不敏感。这些发现进一步表明,MCI功能对于球囊细胞对缺氧的反应是必要的,尽管MCI RET似乎并不需要在这一过程中发挥作用。
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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
7.00
自引率
2.90%
发文量
303
审稿时长
1.0 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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