Assessment of MALDI-TOF MS for the identification of cultural heritage insect pests

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Dikra Hamadouche , Adama Zan Diarra , Fabien Fohrer , Jean-Michel Bérenger , Ahmed Benakhla , Lionel Almeras , Philippe Parola
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Abstract

When heritage properties and contents are threatened by various harmful insects, especially coleopterans, rapid and reliable pest identification is required for effective management. While traditional methods have limitations, MALDI-TOF MS has recently emerged as a groundbreaking identification tool. In this study, our aim was to assess the effectiveness of MALDI-TOF MS in identifying cultural heritage insect pests belonging to the Coleoptera order. To do so, adult, larva, and exuviae from eight species of Coleoptera (Lasioderma serricorne (n = 92), Attagenus smirnovi (n = 87), Trogoderma versicolor (n = 97), Reesa vespulae (n = 74), Anthrenus verbasci (n = 68), Anthrenus pimpinellae (n = 34), Anthrenus flavipes (n = 30), and Thylodrias contractus (n = 19), were subjected to MALDI-TOF MS after optimisation of the sample preparation parameters. This optimisation was based on three key parameters influencing spectrum quality: the choice of body part, the buffer volume for protein extraction, and the method and duration of sample homogenisation. Reproducible intra-species MS spectra for each developmental stage were obtained. The species specificity of MS spectra for each developmental stage supported the use of MALDI- TOF MS for specimen classification. Spectra from 68 samples, including four specimens per species and stage, were added to our homemade reference arthropod MS database (DB). Among spectra classified as being consistent, 97.10% (335/345) of the samples were correctly classified with a relevant identification score. The morphological identification of all adult specimens in the reference MS spectra DB was confirmed by molecular biology, and the sequences were deposited in GenBank. This study is the first demonstration of the effective application of MALDI-TOF MS as a reliable tool for identifying various developmental stages of Coleoptera pests which pose a threat to the cultural heritage of France.

Abstract Image

MALDI-TOF质谱在文物害虫鉴定中的应用评价
当遗产和内容物受到各种有害昆虫,特别是鞘翅目昆虫的威胁时,需要快速、可靠的害虫鉴定,以便进行有效的管理。虽然传统方法有局限性,但MALDI-TOF质谱最近成为一种开创性的鉴定工具。在本研究中,我们的目的是评估MALDI-TOF质谱在鉴定鞘翅目文化遗产害虫方面的有效性。为此,在优化样品制备参数后,对8种鞘翅目昆虫(sericorne Lasioderma 92)、smirnovi Attagenus(87)、Trogoderma versicolor(97)、Reesa vespulae(74)、verbasci(68)、pimpinellae(34)、flavipes(30)和thlodrias contractus(19))的成虫、幼虫和脱毛进行了MALDI-TOF质谱分析。这种优化是基于影响光谱质量的三个关键参数:身体部位的选择、蛋白质提取的缓冲体积、样品均质化的方法和持续时间。获得了每个发育阶段可重复的种内质谱。每个发育阶段的质谱谱的物种特异性支持使用MALDI- TOF质谱进行标本分类。68个样本的光谱(每个物种和阶段4个标本)被添加到我们自制的参考节肢动物MS数据库(DB)中。在被分类为一致的光谱中,97.10%(335/345)的样品被正确分类,并得到相应的识别分数。所有成体标本在参考MS谱DB中的形态鉴定均经分子生物学鉴定,并将序列存入GenBank。这项研究首次证明了MALDI-TOF MS作为一种可靠的工具,有效地应用于鉴定对法国文化遗产构成威胁的鞘翅目害虫的各个发育阶段。
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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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