Effect of ultrasound treatment on the oxidation and conformational structure of myofibrillar protein of beef marinated in red sour soup

IF 7.1 1区农林科学 Q1 Agricultural and Biological Sciences

Meat Science Pub Date : 2025-02-16 DOI:10.1016/j.meatsci.2025.109779

Huaisheng Zheng , Chaobin Huang , Shuhong Liu , Xinghua Chen , Xiaoyu Wang , Ping Hu

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引用次数: 0

Abstract

In this study, we investigated the oxidative and structural effects of red sour soup and ultrasound-assisted marination on beef myofibrillar proteins (MPs). The results demonstrated that marinating with red sour soup could alter MPs structure and oxidative properties, promote MPs aggregation, and increase protein particle size. Compared to traditional static marination, ultrasound-assisted marination significantly elevated protein oxidation levels (P < 0.05), as shown by the increase in carbonyl and reduction in sulfhydryl (P < 0.05). Secondary and tertiary protein structures revealed that ultrasound decreased the α-helix and increased the β-sheet, promoting the exposure of hydrophobic groups. Particle size significantly decreased with increasing ultrasound power (P < 0.05). The SDS-PAGE further illustrated that appropriate ultrasound treatment (320 W) alleviated protein cross-linking and aggregation induced by organic acids. Overall, this study showed that ultrasound ameliorated the effects on protein structure during marinating due to the acidic conditions of red sour soup, offering a theoretical foundation for its application in meat processing.

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来源期刊

Meat Science 工程技术-食品科技

CiteScore

12.60

自引率

9.90%

发文量

282

审稿时长

60 days

期刊介绍： The aim of Meat Science is to serve as a suitable platform for the dissemination of interdisciplinary and international knowledge on all factors influencing the properties of meat. While the journal primarily focuses on the flesh of mammals, contributions related to poultry will be considered if they enhance the overall understanding of the relationship between muscle nature and meat quality post mortem. Additionally, papers on large birds (e.g., emus, ostriches) as well as wild-captured mammals and crocodiles will be welcomed.