Effects of Roselle (Hibiscus sabdariffa Linn.) flower extracts on various inflammatory and bone apposition biomarkers during orthodontic tooth movement: An experimental animal study

Q1 Medicine
Ida Bagus Narmada , Talat Hasan Al-Gunaid , I Gusti Aju Wahju Ardani , Ari Triwardhani , Alida Alida , Tengku Natasha Eleena binti Tengku Ahmad Noor , Reyhan Mahendra Nur , Monika Nilam Suryani , Alivianda Zahrina Saraya , Maria Devitha , Erza Widyarini , Olivia Halim , Wibi Riawan , Putri Cahaya Situmorang , Igo Syaiful Ihsan , Alexander Patera Nugraha
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引用次数: 0

Abstract

Background

Roselle (Hibiscus sabdariffa) flower extract (RFE) can potentially be an adjuvant in orthodontic tooth movement (OTM) in the alveolar bone (AB) by regulating inflammatory response, and bone remodeling through tumor necrosis factor-α (TNF-α), nuclear factor kappa beta (NF-κB), heat shock protein (HSP)-10, HSP-70, alkaline phosphatase (ALP), and osteocalcin expression.

Objective

To investigate TNF-α, NF-κB, HSP-10, HSP-70, ALP, and osteocalcin expression after RFE administration during OTM in the AB in vivo.

Materials and methods

Forty healthy male Wistar rats (Rattus norvegicus) were randomly divided into two groups, OTM group (K) and OTM with RFE group (KP). An 8-mm nickel-titanium closed coil spring and a ligature wire linked from the first upper left molar to the central incisor at a light force of 10 g. The rats received RFE administration using a blunt microneedle and a 0.01-ml dosage on the gingiva sulcus of a molar on days 1, 7, 14, and 21 and were then sacrificed, respectively. The ALP, osteocalcin, NFκβ, TNF-α, HSP-10, and HSP-70 expressions were analyzed immunohistochemically.

Result

The highest HSP-70, NFκβ, and TNF-α expressions on the compression side of the AB found on day 1 were significantly different between the groups (p ≤ 0.05). The highest expressions of ALP and HSP-10 on the tension side were found on day 7. Meanwhile, the highest osteocalcin expression found on day 21 was significantly different between the groups (p ≤ 0.05).

Conclusion

RFE decreased TNF-α, NF-κB, and HSP-70 expression and enhanced osteocalcin and ALP expression in the AB during OTM in Wistar rats (R. novergicus).

Abstract Image

芙蓉花提取物对正畸牙齿运动中各种炎症和骨相关生物标志物的影响:一项实验动物研究
背景:芙芙花提取物(RFE)可能通过肿瘤坏死因子-α (TNF-α)、核因子κ b (NF-κB)、热休克蛋白(HSP)-10、HSP-70、碱性磷酸酶(ALP)和骨钙素的表达调节炎症反应和骨重塑,从而成为牙髓正畸牙齿运动(OTM)的辅助剂。目的观察体外注射RFE后AB组织中TNF-α、NF-κB、HSP-10、HSP-70、ALP和骨钙素的表达。材料与方法健康雄性褐家鼠40只,随机分为OTM组(K)和OTM加RFE组(KP)。一个8毫米的镍钛封闭线圈弹簧和一根结扎线以10克的轻力从左上颌第一磨牙连接到中门牙。大鼠分别于第1、7、14、21天用钝微针和0.01 ml剂量在一磨牙龈沟处给药,然后处死。免疫组化分析ALP、骨钙素、nf - κβ、TNF-α、HSP-10、HSP-70的表达。结果第1天AB受压侧HSP-70、nf - κβ、TNF-α的最高表达量组间差异有统计学意义(p≤0.05)。张力侧ALP和HSP-10在第7天表达最高。第21天骨钙素表达量最高,组间差异显著(p≤0.05)。结论rfe降低了Wistar大鼠OTM时AB组织中TNF-α、NF-κB和HSP-70的表达,提高了骨钙素和ALP的表达。
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来源期刊
CiteScore
4.90
自引率
0.00%
发文量
133
审稿时长
167 days
期刊介绍: Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.
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