Effects of Roselle (Hibiscus sabdariffa Linn.) flower extracts on various inflammatory and bone apposition biomarkers during orthodontic tooth movement: An experimental animal study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-02-22 DOI:10.1016/j.jobcr.2025.02.004

Ida Bagus Narmada , Talat Hasan Al-Gunaid , I Gusti Aju Wahju Ardani , Ari Triwardhani , Alida Alida , Tengku Natasha Eleena binti Tengku Ahmad Noor , Reyhan Mahendra Nur , Monika Nilam Suryani , Alivianda Zahrina Saraya , Maria Devitha , Erza Widyarini , Olivia Halim , Wibi Riawan , Putri Cahaya Situmorang , Igo Syaiful Ihsan , Alexander Patera Nugraha

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引用次数: 0

Abstract

Background

Roselle (Hibiscus sabdariffa) flower extract (RFE) can potentially be an adjuvant in orthodontic tooth movement (OTM) in the alveolar bone (AB) by regulating inflammatory response, and bone remodeling through tumor necrosis factor-α (TNF-α), nuclear factor kappa beta (NF-κB), heat shock protein (HSP)-10, HSP-70, alkaline phosphatase (ALP), and osteocalcin expression.

Objective

To investigate TNF-α, NF-κB, HSP-10, HSP-70, ALP, and osteocalcin expression after RFE administration during OTM in the AB in vivo.

Materials and methods

Forty healthy male Wistar rats (Rattus norvegicus) were randomly divided into two groups, OTM group (K) and OTM with RFE group (KP). An 8-mm nickel-titanium closed coil spring and a ligature wire linked from the first upper left molar to the central incisor at a light force of 10 g. The rats received RFE administration using a blunt microneedle and a 0.01-ml dosage on the gingiva sulcus of a molar on days 1, 7, 14, and 21 and were then sacrificed, respectively. The ALP, osteocalcin, NFκβ, TNF-α, HSP-10, and HSP-70 expressions were analyzed immunohistochemically.

Result

The highest HSP-70, NFκβ, and TNF-α expressions on the compression side of the AB found on day 1 were significantly different between the groups (p ≤ 0.05). The highest expressions of ALP and HSP-10 on the tension side were found on day 7. Meanwhile, the highest osteocalcin expression found on day 21 was significantly different between the groups (p ≤ 0.05).

Conclusion

RFE decreased TNF-α, NF-κB, and HSP-70 expression and enhanced osteocalcin and ALP expression in the AB during OTM in Wistar rats (R. novergicus).

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.