Potential impacts of polyethylene microplastics and heavy metals on Bidens pilosa L. growth: Shifts in root-associated endophyte microbial communities

Abstract

This study investigates the impact of polyethylene (PE) microplastics of varying particle sizes and concentrations on the growth of Bidens pilosa L. and its root-associated microbial communities in cadmium (Cd) and lead (Pb) co-contaminated soil. PE microplastics had a significant impact on plant growth. Notably, at the P05-10 level, root length, root weight, and total biomass exhibited the greatest reductions by 48.9%, 44.1%, and 45.2%, respectively. Furthermore, PE microplastics reduced photosynthetic pigment levels and promoted the accumulation of reactive oxygen species, as indicated by a 264.8% and 57.2% increase in H₂O₂ content in roots and leaves. High-throughput sequencing revealed substantial alterations in the composition of bacterial and fungal communities, with stress-resilient taxa such as Actinobacteria, Verrucomicrobiota, and Rhizophagus exhibiting increased relative abundance. Correlation analyses indicated that variations in soil pH and enzymatic activity influenced microbial community structure, which in turn affected plant physiological responses. Functional predictions using PICRUSt2 and BugBase suggested enhanced oxidative stress tolerance, increased secondary metabolite biosynthesis, and a higher prevalence of stress-resistant phenotypes under conditions of elevated PE concentrations and smaller particle sizes. Overall, this study provides novel insights into the potential effects of microplastics on Bidens pilosa L., particularly in its role as a hyperaccumulator, highlighting its capacity for heavy metal uptake under microplastic exposure.