Integration of comparative cytology, ionome, transcriptome and metabolome provide a basic framework for the response of foxtail millet to Cd stress

Abstract

Apart from directly affecting the growth and development of crops, Cd in the soil can easily enter the human body through the food chain and pose a threat to human health. Therefore, understanding the toxicity of Cd to specific crops and the molecular mechanisms of their response to Cd is essential. In this study, hydroponic experiments were utilized to study the response of foxtail millet to Cd stress through phenotypic investigation, enzyme activity determination, ultrastructure, ionome, transcriptome and metabolome. With the increase in cadmium concentration, both the growth and photosynthetic capacity of foxtail millet seedlings are severely inhibited. The ultrastructure of cells is damaged, cells are deformed, chloroplasts swell and disappear, and cell walls thicken. Cd stress affects the absorption, transport, and redistribution of beneficial metal ions in the seedlings. Multi-omics analysis reveals the crucial roles of glycolysis, glutathione metabolism and phenylpropanoid and lignin biosynthesis pathways in Cd detoxification via energy metabolism, the antioxidant system and cell wall changes. Finally, a schematic diagram of foxtail millet in response to Cd stress was we preliminarily drew. This work provides a basic framework for further revealing the molecular mechanism of Cd tolerance in foxtail millet.