Effects of microplastics accumulation and antibiotics contamination in anaerobic membrane bioreactors for municipal wastewater treatment

Abstract

Municipal wastewater treatment plants are the main collection points for plastics and antibiotics. Anaerobic membrane bioreactor (AnMBR) is one the most potential municipal wastewater treatment technologies. This study evaluated the impact of microplastic (aged polyvinyl chloride, aged PVC, 1.5 g/L), antibiotics (ciprofloxacin, CIP, 100 μg/L) and their interaction effect on AnMBR treatment performance and membrane fouling. Results showed that the inhibition of CIP on AnMBR organic removal and methane production was intensified, owing to the CIP adsorption on aged PVC. The enzyme activities of electron transport (ETS), adenosine triphosphate (ATP) and F420 were also significantly restrained by 47~52% with combined exposure. The combined effects also significantly aggravated the membrane fouling of AnMBR, which shorted the membrane operational period by half due to more soluble microbial products (SMP) secretion. The microbial diversity analyses indicated that aged PVC and CIP addition can accumulate some main anaerobic fermentation bacteria but inhibit the archaea. The abundance of related enzyme in the acetoclastic and hydrogenotrophic methanogenesis decreased with the sole aged PVC and CIP addition and severely inhibited with their combine effect. The absolute abundance mcrA significantly reduced by 92% with combined exposure, validating the negative impact on methanogenic activity. These findings provide valuable insight into the AnMBR implementation in complex wastewater treatment.