Neuropeptide Y at the crossroads of male reproductive functions in a seasonally breeding reptile, Hemidactylus flaviviridis

IF 2.1 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Vishesh Chauhan , Umesh Rai , Mamta Tripathy , Sunil Kumar
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Abstract

Neuropeptide Y (NPY) is known to be a key regulator of reproductive functions across vertebrates. Its role in the modulation of gonadotropin releasing hormone (GnRH) has been extensively explored in mammals and fishes. However, no such report exists in aves, amphibians, or reptiles. Hence, the present study aimed to develop an insight into reproductive phase-dependent expression and role of NPY in the diencephalon region of male wall lizards. Expression of ligand, npy, and its receptor, npyr varied across the annual reproductive cycle of Hemidactylus flaviviridis with the highest expression of both observed during recrudescence. Further, the diencephalon region of recrudescent wall lizards treated with NPY demonstrated an increased mRNA level of gnrh and its receptor, gnrhr. In addition, the current study also elucidates hormonal regulation of diencephalonic npy and npyr wherein neuropeptides like kisspeptin and substance P, the gonadotropin, FSH, as well as sex steroids, dihydrotestosterone (DHT) and 17β-estradiol (E2) inhibited npy and npyr expression in the diencephalon region of wall lizards. With regard to adipokines, leptin stimulated while nesfatin-1 inhibited diencephalonic npy and npyr expression. In conclusion, the current study is the first to present a comprehensive picture of reproductive phase-specific expression pattern, role, and hormonal regulation of neuropeptide Y in the diencephalon region of the male wall lizard, H. flaviviridis.

Abstract Image

神经肽Y在季节性繁殖爬行动物黄半爪虫雄性生殖功能的十字路口。
神经肽Y (NPY)是脊椎动物生殖功能的关键调节因子。其在调节促性腺激素释放激素(GnRH)中的作用已在哺乳动物和鱼类中被广泛探索。然而,在鸟类、两栖动物或爬行动物中没有这样的报道。因此,本研究旨在了解雄性壁虎间脑区NPY的生殖相依赖性表达及其作用。配体npy及其受体npyr的表达在黄绿半指虱的年繁殖周期中有所不同,在复发期两者的表达最高。此外,经NPY处理的复发壁虎间脑区域gnrh及其受体gnrhr的mRNA水平升高。此外,本研究还阐明了间脑npy和npyr的激素调节,其中kisspeptin和substance P等神经肽、促性腺激素、FSH以及性类固醇、双氢睾酮(DHT)和17β-雌二醇(E2)抑制壁蜥间脑区npy和npyr的表达。在脂肪因子方面,瘦素刺激间脑npy和npyr的表达,而nesfatin-1抑制间脑npy和npyr的表达。综上所述,本研究首次全面揭示了雄性黄壁虎间脑区神经肽Y的生殖期特异性表达模式、作用和激素调节。
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来源期刊
CiteScore
5.00
自引率
4.30%
发文量
155
审稿时长
3 months
期刊介绍: Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.
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