Investigation of Clostridium butyricum on atopic dermatitis based on gut microbiota and TLR4/MyD88/ NF-κB signaling pathway.

IF 1.4 4区医学 Q4 ENGINEERING, BIOMEDICAL

Technology and Health Care Pub Date : 2025-01-17 DOI:10.1177/09287329241301680

Xiaojing Yang, Qian Zhao, Xing Wang, Yiming Zhang, Jingyue Ma, Yuanjun Liu, Huiping Wang

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引用次数: 0

Abstract

Background: Probiotics, as common regulators of the gut microbiota, have been used in research to alleviate clinical symptoms of atopic dermatitis (AD).

Objective: Our research team has previously identified a potential relieving effect of Clostridium butyricum on the treatment of AD, but the specific mechanism of how Clostridium butyricum alleviates AD has not yet been confirmed.

Methods: In this study, we explored the relieving effect of Clostridium butyricum on AD through in vivo and in vitro experiments. AD mice induced by 2,4-dinitrofluorobenzene (DNFB) were orally administered with 1 × 10⁸ CFU of Clostridium butyricum for three consecutive weeks.

Results: Oral administration of Clostridium butyricum reduced ear swelling, alleviated back skin lesions, decreased mast cell and inflammatory cell infiltration, and regulated the levels of inflammation-related cytokines. Clostridium butyricum activated the intestinal immune system through the TLR4/MyD88/NF-κB signaling pathway, suppressed the expression of inflammatory factors IL-10 and IL-13, and protected the damaged intestinal mucosa.

Conclusion: Clostridium butyricum administration improved the diversity and abundance of the gut microbiota, enhanced the functionality of the immune system, and protected the epidermal barrier.

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基于肠道菌群和TLR4/MyD88/ NF-κB信号通路的丁酸梭菌治疗特应性皮炎的研究

背景：益生菌作为肠道微生物群的常见调节剂，已被用于研究缓解特应性皮炎（AD）的临床症状。目的：本课课组前期已发现丁酸梭菌对AD的治疗具有潜在的缓解作用，但丁酸梭菌缓解AD的具体机制尚未得到证实。方法：本研究通过体内和体外实验探讨丁酸梭菌对AD的缓解作用。以2,4-二硝基氟苯（DNFB）致AD小鼠，连续3周口服1 × 108 CFU的丁酸梭菌。结果：口服丁酸梭菌可减轻耳部肿胀，减轻背部皮肤病变，减少肥大细胞和炎症细胞浸润，调节炎症相关细胞因子水平。丁酸梭菌通过TLR4/MyD88/NF-κB信号通路激活肠道免疫系统，抑制炎症因子IL-10和IL-13的表达，保护受损的肠黏膜。结论：丁酸梭菌可提高肠道菌群的多样性和丰度，增强免疫系统功能，保护表皮屏障。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Technology and Health Care HEALTH CARE SCIENCES & SERVICES-ENGINEERING, BIOMEDICAL

CiteScore

2.10

自引率

6.20%

发文量

282

审稿时长

>12 weeks

期刊介绍： Technology and Health Care is intended to serve as a forum for the presentation of original articles and technical notes, observing rigorous scientific standards. Furthermore, upon invitation, reviews, tutorials, discussion papers and minisymposia are featured. The main focus of THC is related to the overlapping areas of engineering and medicine. The following types of contributions are considered: 1.Original articles: New concepts, procedures and devices associated with the use of technology in medical research and clinical practice are presented to a readership with a widespread background in engineering and/or medicine. In particular, the clinical benefit deriving from the application of engineering methods and devices in clinical medicine should be demonstrated. Typically, full length original contributions have a length of 4000 words, thereby taking duly into account figures and tables. 2.Technical Notes and Short Communications: Technical Notes relate to novel technical developments with relevance for clinical medicine. In Short Communications, clinical applications are shortly described. 3.Both Technical Notes and Short Communications typically have a length of 1500 words. Reviews and Tutorials (upon invitation only): Tutorial and educational articles for persons with a primarily medical background on principles of engineering with particular significance for biomedical applications and vice versa are presented. The Editorial Board is responsible for the selection of topics. 4.Minisymposia (upon invitation only): Under the leadership of a Special Editor, controversial or important issues relating to health care are highlighted and discussed by various authors. 5.Letters to the Editors: Discussions or short statements (not indexed).