Multi-technique-based electrochemical sensing of lipoarabinomannan (LAM) antigen as a biomarker for early-stage tuberculosis diagnosis.

IF 2.9 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanotechnology Pub Date : 2025-03-03 DOI:10.1088/1361-6528/adb7ea

Dinesh R Rotake, Shubham C Anjankar, Shiv Govind Singh

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引用次数: 0

Abstract

Tuberculosis (TB) remains a pressing global health challenge, necessitating precise and reliable biomarkers for early detection. Lipoarabinomannan (LAM), an FDA-approved biomarker (Monoclonal Antibody-MBS320597), holds significant potential due to its association with theMycobacterium tuberculosiscell wall. This study systematically evaluates LAM concentrations ranging from 1 pg ml^-1to 6 ng ml^-1using square wave voltammetry analysis, achieving an exceptional limit of detection of 0.077 pg ml^-1. A comprehensive review of current diagnostics highlights critical gaps, including limitations in speed and accuracy, underscoring the urgency for advanced methodologies. In this study, LAM's performance is assessed by analyzing spiked urine samples, demonstrating its high sensitivity, specificity, and reliability as an early-stage TB biomarker. By comparing findings with existing diagnostic tools and addressing identified limitations, this study emphasizes LAM's potential to transform TB diagnostic strategies. These results contribute to global efforts to improve early detection, enhance patient outcomes, and pave the way for future advancements in TB diagnostics.

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多技术电化学检测脂arabinman聚糖（LAM）抗原作为早期结核诊断的生物标志物。

结核病仍然是一项紧迫的全球卫生挑战，需要精确可靠的生物标志物进行早期检测。lipoarabinmanannan （LAM）是fda批准的生物标志物（单克隆抗体- mbs320597），由于其与结核分枝杆菌细胞壁的相关性而具有重要的潜力。本研究使用方波伏安（SWV）分析系统地评估了1 pg/mL至6 ng/mL范围内的LAM浓度，实现了0.077 pg/mL的异常检测限（LOD）。对现有诊断方法的全面审查突出了关键差距，包括速度和准确性方面的限制，强调了采用先进方法的紧迫性。在这项研究中，LAM通过分析加标尿液样本来评估其性能，证明其作为早期结核病生物标志物的高灵敏度、特异性和可靠性。通过将研究结果与现有诊断工具进行比较，并解决已确定的局限性，本研究强调了LAM改变结核病诊断策略的潜力。这些结果有助于全球努力改善早期发现，改善患者预后，并为结核病诊断的未来进展铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanotechnology 工程技术-材料科学：综合

CiteScore

7.10

自引率

5.70%

发文量

820

审稿时长

2.5 months

期刊介绍： The journal aims to publish papers at the forefront of nanoscale science and technology and especially those of an interdisciplinary nature. Here, nanotechnology is taken to include the ability to individually address, control, and modify structures, materials and devices with nanometre precision, and the synthesis of such structures into systems of micro- and macroscopic dimensions such as MEMS based devices. It encompasses the understanding of the fundamental physics, chemistry, biology and technology of nanometre-scale objects and how such objects can be used in the areas of computation, sensors, nanostructured materials and nano-biotechnology.