Multi-technique-based electrochemical sensing of lipoarabinomannan (LAM) antigen as a biomarker for early-stage tuberculosis diagnosis.

IF 2.9 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanotechnology Pub Date : 2025-02-19 DOI:10.1088/1361-6528/adb7ea

Dinesh Ramkrushna Rotake, Shubham C Anjankar, Shiv Govind Singh

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引用次数: 0

Abstract

Tuberculosis (TB) remains a pressing global health challenge, necessitating precise and reliable biomarkers for early detection. Lipoarabinomannan (LAM), an FDA-approved biomarker (Monoclonal Antibody-MBS320597), holds significant potential due to its association with the Mycobacterium tuberculosis cell wall. This study systematically evaluates LAM concentrations ranging from 1 pg/mL to 6 ng/mL using Square Wave Voltammetric (SWV) analysis, achieving an exceptional detection limit (LOD) of 0.077 pg/mL. A comprehensive review of current diagnostics highlights critical gaps, including limitations in speed and accuracy, underscoring the urgency for advanced methodologies. In this study, LAM's performance is assessed by analyzing spiked urine samples, demonstrating its high sensitivity, specificity, and reliability as an early-stage TB biomarker. By comparing findings with existing diagnostic tools and addressing identified limitations, this study emphasizes LAM's potential to transform TB diagnostic strategies. These results contribute to global efforts to improve early detection, enhance patient outcomes, and pave the way for future advancements in TB diagnostics.

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来源期刊

Nanotechnology 工程技术-材料科学：综合

CiteScore

7.10

自引率

5.70%

发文量

820

审稿时长

2.5 months

期刊介绍： The journal aims to publish papers at the forefront of nanoscale science and technology and especially those of an interdisciplinary nature. Here, nanotechnology is taken to include the ability to individually address, control, and modify structures, materials and devices with nanometre precision, and the synthesis of such structures into systems of micro- and macroscopic dimensions such as MEMS based devices. It encompasses the understanding of the fundamental physics, chemistry, biology and technology of nanometre-scale objects and how such objects can be used in the areas of computation, sensors, nanostructured materials and nano-biotechnology.