Genomic characterisation of emerging Enterococcus faecium vanA types from 2013 to 2020 in an Australian public hospital

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2025-02-17 DOI:10.1016/j.jgar.2025.02.006

Ronan F. O'Toole , Vitalie Covalciuc , Ana Cooke , Gaetan Thilliez , Emma K. Finlay , Kelvin W.C. Leong , Vanessa Farthing , Sebastiaan J. van Hal

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引用次数: 0

Abstract

Objective

High rates of resistance to vancomycin are now being reported among invasive isolates of Enterococcus faecium, a major cause of healthcare-associated infections globally. The objective of this study was to generate a better understanding of emerging vanA sequence types (ST) of the pathogen.

Methods

A temporal analysis of isolates collected from 2013 to 2020 at the Royal Prince Alfred Hospital, a large Australian hospital, was performed using genome sequencing. Relative frequencies of multi-locus ST, antibiotic resistance markers, and virulence genes were determined.

Results

ST1421 was the dominant vanA ST from 2014 to 2018. ST1424, which was not evident in the 2013 and 2014 isolates, emerged in 2016 and became the dominant vanA type in 2020 (65% of isolates). vanA ST80 was less common among the Royal Prince Alfred Hospital vanA isolates. Direct comparison of 120 genomes of each ST revealed significantly higher encoded resistance to aminocyclitols (e.g. spectinomycin) and folate-pathway antagonists (e.g. trimethoprim) in ST1421 and ST1424 compared to ST80. Furthermore, significantly higher carriage of enterococcal virulence genes ecbA (E. faecium collagen binding protein A) and hylEfm (glycosyl hydrolase) was found in ST1421 and ST1424 than in ST80.

Conclusions

Newer vanA ST ST1421 and ST1424 harboured several antibiotic resistance loci and virulence genes at significantly higher levels than those observed in ST80. Ongoing genomic surveillance is warranted for the detection of new variants of E. faecium and characterisation of their encoded resistance and virulence.

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2013 - 2020年澳大利亚一家公立医院中新出现的屎肠球菌vanA型的基因组特征

目的：据报道，在侵入性粪肠球菌分离株中，万古霉素耐药率很高，这是全球卫生保健相关感染的主要原因。本研究的目的是为了更好地了解新出现的病原体的vanA序列类型。方法：采用基因组测序技术对2013-2020年在澳大利亚大型医院阿尔弗雷德亲王医院（Royal Prince Alfred Hospital， RPAH）采集的分离株进行时间分析。测定了多位点序列类型、抗生素耐药标记和毒力基因的相对频率。结果：2014 - 2018年，ST1421为主要的vanA序列类型。2013年和2014年分离株中不明显的ST1424型在2016年出现，并在2020年成为主要的vanA型（占分离株的65%）。在RPAH分离株中，vanA ST80较少见。对每种序列类型的120个基因组的直接比较显示，与ST80相比，ST1421和ST1424对氨基环醇（如大观霉素）和叶酸途径拮抗剂（如甲氧苄啶）的编码抗性显著更高。此外，ST1421和ST1424中携带的肠球菌毒力基因ecbA （E. faecium collagen binding protein A）和hylEfm （glycosyl hydrolase）显著高于ST80。结论：较新的vanA序列型ST1421和ST1424含有多个抗生素耐药位点和毒力基因，其水平明显高于ST80。持续的基因组监测是必要的，以检测新的粪肠杆菌变异和表征其编码的抗性和毒力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.