Humoral immunity to lung antigens early post-transplant confers risk for chronic lung allograft dysfunction.

Abstract

Background: Autoantibodies and de novo donor HLA-specific antibodies (dnDSA) may contribute to chronic lung allograft dysfunction (CLAD). However, the breadth of reactivities against self-antigens and their association with CLAD has been underexamined. In a single-centre study, we screened lung transplant (LTx) recipients for novel autoantibodies at transplant and 6 months post-LTx, assessed dnDSA exposure, and tested their relationship with CLAD-free survival.

Methods: Serum samples were collected from 89 crossmatch-negative bilateral lung transplant recipients at the time of LTx and 6 months post-LTx, prior to a CLAD diagnosis, for autoantibody screening using a custom antigen microarray optimized for IgM and IgG detection.

Results: Patients who developed CLAD by 5 years post-LTx demonstrated a decrease in average IgG reactivity, but no decrease in IgM reactivity when measured at 6 months post-LTx. IgG anti-tropoelastin, SP-D, and thyroglobulin autoantibodies were significantly elevated 6 months post-LTx in patients who developed CLAD by 5 years, compared to those who remained CLAD-free at 5 years. In contrast, patients who remained CLAD-free at 5 years had elevated levels of IgG anti-CENP-B at both timepoints and PM/SCL100 at 6 months post-LTx, suggesting these may confer protection. Exposure to autoantibodies against lung-enriched targets, as opposed to ubiquitous antigens, and dnDSA conferred increased CLAD risk.

Conclusions: We have identified novel autoantibodies associated with CLAD-free survival. Our results bolster the independent relationship between autoantibodies and CLAD. We also identified autoantibody signatures that are associated with a marked increase in CLAD risk. Exposure to lung-enriched targets and dnDSA may have a reciprocal amplifying effect that lies on a tissue-specific mechanistic pathway leading to CLAD.