Dan Wang, Xiaojun Zhu, Buhe Siqin, Chao Ren, Ming Chang, Ligang Bai
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引用次数: 0
Abstract
Background: Urethral stricture (US) is a common condition that considerably affects patients' quality of life.
Objectives: This study aimed to explore the adoption value of the extracellular vesicle (EV)- small intestinal submucosa (SIS) complex in the repair of USs.
Methods: EVs were extracted from healthy male New Zealand white rabbits, and SIS was prepared using peracetic acid (PAA) oxidation and decellularization. The morphology and particle size of the prepared EV-SIS complex were evaluated using electron microscopy and qNano nanoparticle analyzer, and the labeled proteins of EVs were detected using Western blot method. EV-SIS the complex was implanted in a rabbit model of US, and urodynamic parameters were assessed.
Results: The EV-SIS complex displayed a full morphology, intact membrane structure, and uniform particle size. The protein concentration of EVs in the complex was approximately 0.351 µg/µL, with a yield of approximately 1.86 µg/106 cells. The complex exhibited remarkable repair effects in the rabbit model of US, with bladder capacity, maximal urethral pressure, and minimal urethral pressure all markedly superior to those in the US group (P < 0.05).
Conclusion: The EV-SIS complex demonstrates potential clinical value in the repair of USs, improving urodynamic parameters, and offering a promising therapeutic option for patients with US.
期刊介绍：
The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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