{"title":"Pre-alignment of fibroblasts induced by micropatterning and cyclic stretching techniques accelerates wound healing.","authors":"Ryota Toyohara, Yanan Lan, Yu Chen, Takaya Yoriki, Toshiro Ohashi","doi":"10.1177/09592989241305522","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Wound healing proceeds through a complex collaborative process. It has been shown that during the intermediate phase of the wound healing process, fibroblasts migrate into wound area and contract to contribute to the closure of the wound area. Moreover, previous studies have shown that fibroblast alignment was observed on the mature stage of wound scar. These studies clearly indicate that fibroblasts play a critical role in wound healing process, however, the whole mechanism of wound healing remains still unclear.</p><p><strong>Objective: </strong>Fibroblasts are pre-aligned to evaluate the effect of cell alignment on cell migration rate.</p><p><strong>Methods: </strong>The cell alignment was accomplished by PDMS microstamping with fibronectin and application of cyclic stretching. Wound was created by physical scratching and then the wound closure rate was measured.</p><p><strong>Results: </strong>The pre-aligned cells perpendicular to the direction of scratched wound exhibited significantly higher migration rate, compared to non-aligned control cells. Moreover, pre-aligned cells with thick actin filaments by cyclic stretching migrated faster than those with less development of actin filament structures by microstamping.</p><p><strong>Conclusion: </strong>The wound closure can be accelerated by the adequate alignment of fibroblasts as well as the development of actin filament structures.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"9592989241305522"},"PeriodicalIF":1.0000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bio-medical materials and engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/09592989241305522","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Wound healing proceeds through a complex collaborative process. It has been shown that during the intermediate phase of the wound healing process, fibroblasts migrate into wound area and contract to contribute to the closure of the wound area. Moreover, previous studies have shown that fibroblast alignment was observed on the mature stage of wound scar. These studies clearly indicate that fibroblasts play a critical role in wound healing process, however, the whole mechanism of wound healing remains still unclear.
Objective: Fibroblasts are pre-aligned to evaluate the effect of cell alignment on cell migration rate.
Methods: The cell alignment was accomplished by PDMS microstamping with fibronectin and application of cyclic stretching. Wound was created by physical scratching and then the wound closure rate was measured.
Results: The pre-aligned cells perpendicular to the direction of scratched wound exhibited significantly higher migration rate, compared to non-aligned control cells. Moreover, pre-aligned cells with thick actin filaments by cyclic stretching migrated faster than those with less development of actin filament structures by microstamping.
Conclusion: The wound closure can be accelerated by the adequate alignment of fibroblasts as well as the development of actin filament structures.
期刊介绍：
The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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