Rapid and accurate detection of huanglongbing in citrus by elasticity testing using a piezoelectric finger†

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Pawan Rao, Shu Huang, Cheryl M. Armstrong, Joseph Capobianco, YongPing Duan, Wei-Heng Shih and Wan Y. Shih
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Abstract

Rapid and sensitive detection of citrus huanglongbing (HLB) is critical for the control of this devastating disease. In this study, we have evaluated using a piezoelectric finger (PEF) with a 0.4 mm probe to measure the elastic modulus of a leaf to detect HLB in four different species of citrus including grapefruit (GFT), pumelo (PUM), lemon (LEM), and Valencia orange (VAL). Diseased citrus leaves were harvested from trees testing positive for the presence of Candidatus Liberibacter asiaticus (Las), the causal agent of HLB, and included both symptomatic leaves, which were blotchy mottle or yellowing and asymptomatic leaves, which did not display outward symptoms. Healthy leaves were harvested from trees testing negative for Las. The results indicated that the PEF elastic modulus test exhibited an overall 94% sensitivity and 90% specificity against the Las status of the trees for all four citrus types combined. Comparative quantitative real-time polymerase chain reaction (qPCR) tests on the same leaves showed an overall 89% sensitivity and 100% specificity against the Las status of the trees. While a Cohen–Kappa coefficient of 0.81 was obtained between the PEF and qPCR predictions, suggesting a “strong” agreement between the PEF and qPCR tests, a more detailed examination indicated that PEF was more sensitive overall in detecting the Las positive trees than qPCR, particularly from asymptomatic leaves for which PEF was 96% sensitive versus 78% sensitive by qPCR, indicating the potential of using PEF for early detection of HLB.

Abstract Image

利用压电手指弹性试验快速准确地检测柑橘中的黄龙冰。
快速、灵敏地检测黄龙病对防治黄龙病具有重要意义。在这项研究中,我们评估了使用压电手指(PEF)与0.4 mm探针测量叶片的弹性模量,以检测四种不同种类柑橘的HLB,包括葡萄柚(GFT),柚子(PUM),柠檬(LEM)和瓦伦西亚橙(VAL)。从检测出HLB致病因子亚洲解放候选菌(Candidatus Liberibacter asiaticus, Las)存在阳性的树木上采集患病的柑橘叶片,包括有斑点斑纹或发黄的症状叶片和没有表现出外部症状的无症状叶片。健康的叶子是从对Las检测呈阴性的树上收获的。结果表明,PEF弹性模量测试对所有4种柑橘类型的Las状态的总体敏感性为94%,特异性为90%。比较定量实时聚合酶链反应(qPCR)在相同叶片上的检测结果显示,对Las状态的敏感性为89%,特异性为100%。虽然PEF和qPCR预测之间的科恩-卡帕系数为0.81,表明PEF和qPCR测试之间存在“强”一致性,但更详细的检查表明,PEF在检测Las阳性树方面总体上比qPCR更敏感,特别是在无症状叶片中,PEF的敏感性为96%,而qPCR的敏感性为78%,这表明使用PEF早期检测HLB的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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