Spectrum-Effect Relationship Between Fingerprints and Anti-Inflammatory and Antitussive Activities of Raw and Processed Mume Fructus Extracts

IF 1.8 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Zhenguo Lv, Wenhan Lin, Omachi Daniel Ogaji, Yang Liu, Shuting Zhou, Yameng Zhu, Xiwei Wu, Peng Zhao, Tiantian Wu, Caixia Li, Fanjiao Zuo, Huizi Ouyang, Jun He
{"title":"Spectrum-Effect Relationship Between Fingerprints and Anti-Inflammatory and Antitussive Activities of Raw and Processed Mume Fructus Extracts","authors":"Zhenguo Lv,&nbsp;Wenhan Lin,&nbsp;Omachi Daniel Ogaji,&nbsp;Yang Liu,&nbsp;Shuting Zhou,&nbsp;Yameng Zhu,&nbsp;Xiwei Wu,&nbsp;Peng Zhao,&nbsp;Tiantian Wu,&nbsp;Caixia Li,&nbsp;Fanjiao Zuo,&nbsp;Huizi Ouyang,&nbsp;Jun He","doi":"10.1002/bmc.70037","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Mume Fructus (MF), a representative substance in the field of medicine-food homology, has been extensively utilized in clinical treatments and daily diets for its raw and processed forms. This study aimed to establish the spectrum-effect relationship between ultra–high-performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) fingerprints and anti-inflammatory and antitussive activities of raw and processed MF extracts. In UHPLC-Q-TOF-MS/MS fingerprints, a total of 21 common peaks were identified. Bioactivity assays demonstrated that the raw and processed MF extracts exhibited varying degrees of anti-inflammatory and antitussive effects. Subsequently, the relevant pharmacologically active ingredients were screened by grey relation analysis and partial least squares regression. The results showed that nine components were associated with anti-inflammatory effects, namely, citric acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, fumaric acid, gallic acid, neochlorogenic acid, chlorogenic acid, and ononin. Additionally, three components were linked to antitussive activity: amygdalin, syringic acid, and succinic acid, respectively. This work developed a model combining UHPLC-Q-TOF-MS/MS fingerprints with anti-inflammatory and antitussive activities to study the spectrum-effect relationship of raw and processed MF extracts. The findings provide a reference for the discovery of bioactive components and contribute to the clinical rationalization of medications.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70037","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Mume Fructus (MF), a representative substance in the field of medicine-food homology, has been extensively utilized in clinical treatments and daily diets for its raw and processed forms. This study aimed to establish the spectrum-effect relationship between ultra–high-performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) fingerprints and anti-inflammatory and antitussive activities of raw and processed MF extracts. In UHPLC-Q-TOF-MS/MS fingerprints, a total of 21 common peaks were identified. Bioactivity assays demonstrated that the raw and processed MF extracts exhibited varying degrees of anti-inflammatory and antitussive effects. Subsequently, the relevant pharmacologically active ingredients were screened by grey relation analysis and partial least squares regression. The results showed that nine components were associated with anti-inflammatory effects, namely, citric acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, fumaric acid, gallic acid, neochlorogenic acid, chlorogenic acid, and ononin. Additionally, three components were linked to antitussive activity: amygdalin, syringic acid, and succinic acid, respectively. This work developed a model combining UHPLC-Q-TOF-MS/MS fingerprints with anti-inflammatory and antitussive activities to study the spectrum-effect relationship of raw and processed MF extracts. The findings provide a reference for the discovery of bioactive components and contribute to the clinical rationalization of medications.

梅子生、加工提取物指纹图谱与抗炎、止咳活性的光谱效应关系
梅子(Mume Fructus, MF)是药食同源领域的代表物质,其原料和加工形式已广泛应用于临床治疗和日常饮食中。本研究旨在建立超高效液相色谱-四极杆串联飞行时间质谱(UHPLC-Q-TOF-MS/MS)指纹图谱与MF提取物的抗炎、止咳活性之间的谱效关系。UHPLC-Q-TOF-MS/MS指纹图谱共鉴定出21个共同峰。生物活性分析表明,原料和加工的MF提取物具有不同程度的抗炎和止咳作用。通过灰色关联分析和偏最小二乘回归对相关药理活性成分进行筛选。结果表明,柠檬酸、异绿原酸B、异绿原酸A、异绿原酸C、富马酸、没食子酸、新绿原酸、绿原酸和芥子苷等9种成分具有抗炎作用。此外,还有三种成分与止咳活性有关:苦杏仁苷、丁香酸和琥珀酸。本文建立了UHPLC-Q-TOF-MS/MS指纹图谱与抗炎、止咳活性相结合的模型,研究了中草药提取物和炮制提取物的光谱效应关系。研究结果可为发现生物活性成分提供参考,有助于临床合理用药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信