Targeting LncRNA-Vof16: A Novel Therapeutic Strategy for Neuropathic Pain Relief

IF 5 1区医学 Q1 NEUROSCIENCES

CNS Neuroscience & Therapeutics Pub Date : 2025-02-21 DOI:10.1111/cns.70241

Xiuying He, David H. Mauki, Xiaoming Zhao, Songyu Dai, Huisi Yang, Yuexiang Zheng, Qingjie Xia, Rurong Wang, Tinghua Wang

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Abstract

Aims

Neuropathic pain (NP) is a debilitating condition characterized by chronic pain resulting from nerve damage or lesion. Despite the ongoing efforts of clinically defining NP, its distinctive mechanisms that lead to various NP phenotypes remain unresolved.

Methods

Using a spared nerve injury (SNI) model, we investigated the mechanisms underlying the development of NP caused by injury in the peripheral nerves. With CRISPR-Cas9-mediated knockout and virus-mediated overexpression strategies, we investigated the role of LncRNA Vof16 (abbreviated as Vof16) during SNI-induced NP.

Results

Our results revealed that SNI led to the downregulation of Vof16 expression in spinal dorsal horn (SDH) of lumbar enlargement. This was evidently confirmed when we disrupted the expression of Vof16 in SNI rats of which we observed exacerbation of hyperalgesia; while overexpressing it alleviated the pain.

Conclusion

Our findings suggest that Vof16 plays a crucial role in maintaining normal sensory function in healthy states and a protective shield against NP following peripheral nerve injury. We therefore propose Vof16 as a new therapeutic target for alleviating NP.

Abstract Image

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靶向LncRNA-Vof16：神经性疼痛缓解的新治疗策略

目的神经性疼痛是一种以神经损伤或病变引起的慢性疼痛为特征的衰弱性疾病。尽管临床上正在努力定义NP，但其导致各种NP表型的独特机制仍未得到解决。方法采用余留神经损伤（SNI）模型，探讨周围神经损伤致NP发生的机制。通过crispr - cas9介导的敲除和病毒介导的过表达策略，我们研究了LncRNA Vof16（简称Vof16）在sni诱导NP中的作用。结果SNI可导致大鼠腰大鼠脊背角（SDH）中Vof16表达下调。当我们在SNI大鼠中破坏Vof16的表达时，我们观察到痛觉过敏加剧，这一点得到了明显的证实；而过度表达则减轻了痛苦。结论Vof16在维持健康状态下的正常感觉功能和周围神经损伤后NP的保护屏障中起重要作用。因此，我们提出Vof16作为缓解NP的新治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

CNS Neuroscience & Therapeutics 医学-神经科学

CiteScore

7.30

自引率

12.70%

发文量

240

审稿时长

2 months

期刊介绍： CNS Neuroscience & Therapeutics provides a medium for rapid publication of original clinical, experimental, and translational research papers, timely reviews and reports of novel findings of therapeutic relevance to the central nervous system, as well as papers related to clinical pharmacology, drug development and novel methodologies for drug evaluation. The journal focuses on neurological and psychiatric diseases such as stroke, Parkinson’s disease, Alzheimer’s disease, depression, schizophrenia, epilepsy, and drug abuse.