Flow Velocity Modulates Growth, Oxidative Stress, and Transcriptomic Responses in Spotted Sea Bass (Lateolabrax maculatus)

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Shuo Li, Yuyan Liu, Qian Wang, Zhiwen Zhu, Weijing Li, Chen Li, Fan Fei, Baoliang Liu, Changwei Shao
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引用次数: 0

Abstract

Flow velocity is a critical environmental factor influencing the growth, energy metabolism, and physiological health of aquaculture species. This study investigated the physiological and molecular responses of spotted sea bass (Lateolabrax maculatus) under experimental conditions simulating flow velocities typical of land-based recirculating aquaculture systems (RAS) and deep-sea cage systems. High flow velocities (HFV, 0.35–0.65 body lengths per second [BL/s]) enhanced growth performance compared to low flow velocity (LFV, 2.28–2.85 BL/s) conditions. Histological analysis revealed reduced hepatic lipid accumulation under HFV, while LFV promoted lipid storage. Serum analyses showed elevated antioxidant enzyme activity in the LFV group but higher oxidative stress markers in the HFV group. Transcriptomic profiling identified foxo3 as a key regulatory hub orchestrating metabolic and oxidative stress adaptations. Genes associated with oxidative damage repair, lipid catabolism, and glucose metabolism were significantly enriched under hydrodynamic stress. Enrichment of the FoxO signaling pathway highlighted its central role in mediating oxidative stress mitigation and energy mobilization. These findings demonstrate the dual effects of flow velocity, where higher velocities promote growth and metabolic activity at the cost of oxidative stress, and lower velocities conserve energy while maintaining oxidative stability. Tailored flow velocity conditions can optimize fish welfare and productivity across aquaculture systems. Future studies should investigate the systemic effects of hydrodynamic stress using multi-omics approaches to advance sustainable aquaculture practices.

流速调节斑点海鲈鱼生长、氧化应激和转录组反应
流速是影响水产养殖物种生长、能量代谢和生理健康的重要环境因子。在模拟陆基循环水养殖系统(RAS)和深海网箱养殖系统典型流速的实验条件下,研究了斑点黑鲈(Lateolabrax maculatus)的生理和分子反应。高流速(HFV, 0.35-0.65体长/秒[BL/s])比低流速(LFV, 2.28-2.85 BL/s)条件下生长性能更好。组织学分析显示HFV降低了肝脏脂质积累,而LFV促进了脂质储存。血清分析显示,LFV组抗氧化酶活性升高,而HFV组氧化应激标志物升高。转录组学分析鉴定foxo3是协调代谢和氧化应激适应的关键调控中心。在水动力胁迫下,与氧化损伤修复、脂质分解代谢和葡萄糖代谢相关的基因显著富集。FoxO信号通路的富集突出了其在介导氧化应激缓解和能量动员中的核心作用。这些发现证明了流速的双重作用,其中较高的流速以氧化应激为代价促进生长和代谢活动,而较低的流速在保持氧化稳定性的同时节约能量。量身定制的流速条件可以优化整个水产养殖系统的鱼类福利和生产力。未来的研究应利用多组学方法研究水动力应力的系统效应,以促进可持续水产养殖实践。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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