Effect of Dalbergiella welwitschi alkaloid-rich leaf extracts on testicular damage in streptozotocin-induced diabetic rats

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-02-20 DOI:10.1007/s10735-025-10366-x

B. O. Ajiboye, F. I. Ayemoni, C. D. Famusiwa, O. E. Lawal, J. A. Falode, S. A. Onikanni, M. F. Akhtar, S. Gupta, B. E. Oyinloye

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Abstract

Diabetes mellitus is a chronic disease affecting young and old, even though it can be managed with orthodox medicine, which has a series of side effects. Therefore, Dalbergiella welwitschi is one of the medicinal plants that is commonly used for the management of diabetes mellitus and its associated complications. Hence, this study was designed to assess the testicular-protective ability of alkaloid-rich leaf extract of D. welwitschi in streptozotocin-induced type 2 diabetic rats D. welwitshii leaf alkaloid-rich extract was obtained using standard procedure. Streptozotocin was injected into the experimental animals intraperitoneally at a dose of 45 mg/kg body weight to induce type 2 diabetes mellitus. Prior to this, the animals were given 20% (w/v) fructose for one week. Thus, the animals were grouped into five (n = 8), comprising of un-induced rats (NC), diabetic control (DC), diabetic rats treated with low (50 mg/kg body weight) and high (100 mg/kg body weight) doses of D. welwitschi alkaloid-rich leaf extracts (i.e., DWL and DWH respectively) and 200 mg/kg body weight dose of metformin (MET). The animals were sacrificed on the 21st day, blood and testis were harvested and used for the determination of ions (Fe, Cu and Zn), sialic acid, some hormones (testosterone, luteinizing and follicle stimulating), oxidative stress biomarkers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione (GSH)] as well as histological examination. In addition, the results show that diabetic rats placed on DWL, and DWH significantly (p < 0.05) decreased ion levels (Fe, Cu and Zn) and ameliorated oxidative stress biomarkers such as MDA, SOD, CAT, GPx, GST, and GSH. These were supported by the histological examination by improving testicular-protective effects in diabetic rats administered DWL, and DWH. Therefore, it is that assume that the alkaloid-rich leaf extracts of D. welwitschi may offer potential benefits in the treatment of diabetic testicular dysfunction.

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富生物碱黄檀叶提取物对链脲佐菌素致糖尿病大鼠睾丸损伤的影响

糖尿病是一种影响年轻人和老年人的慢性疾病，尽管它可以用传统医学来治疗，但它有一系列的副作用。因此，黄檀是治疗糖尿病及其相关并发症的常用药用植物之一。因此，本研究旨在评价富生物碱的韦氏叶提取物对链脲佐菌素诱导的2型糖尿病大鼠的睾丸保护作用。实验动物以45 mg/kg体重腹腔注射链脲佐菌素诱导2型糖尿病。在此之前，动物被给予20% （w/v）的果糖一周。将实验动物分为5组（n = 8），分别为未诱导大鼠（NC）、糖尿病对照大鼠（DC）、低剂量（50 mg/kg体重）和高剂量（100 mg/kg体重）威氏富生物碱叶提取物（分别为DWL和DWH）和200 mg/kg体重二甲双胍（MET）治疗的糖尿病大鼠。第21天处死动物，采集血液和睾丸，测定离子（铁、铜、锌）、唾液酸、部分激素（睾酮、促黄体生成素和促卵泡）、氧化应激生物标志物[丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽s转移酶（GST）和谷胱甘肽（GSH）]，并进行组织学检查。此外，结果表明，糖尿病大鼠给予DWL和DWH显著（p < 0.05）降低离子水平（Fe、Cu和Zn），改善氧化应激生物标志物（MDA、SOD、CAT、GPx、GST和GSH）。给药DWL和DWH改善糖尿病大鼠睾丸保护作用的组织学检查支持了这一点。因此，我们认为，韦氏叶提取物富含生物碱，可能对糖尿病睾丸功能障碍的治疗有潜在的益处。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.