Balancing biomonitoring and conservation: A non-lethal method to extract microplastics from the sea anemone Bunodosoma cangicum

IF 5.3 3区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Marine pollution bulletin Pub Date : 2025-02-21 DOI:10.1016/j.marpolbul.2025.117685

Leonardo Mario Siqueira Morais , Victória Akim , Daiane Aviz , David Chelazzi , Alessandra Cincinelli , Laura Sforzi , Tommaso Giarrizzo , José Eduardo Martinelli Filho

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引用次数: 0

Abstract

The sea anemone Bunodosoma cangicum is a potential biomonitor species of microplastic (MP) contamination. However, current MP extraction methods for this species, and most organisms, are lethal to the specimens. This poses the risk of negatively impact on local populations, which are already under considerable anthropogenic pressures. Therefore, this study aimed to evaluate a non-lethal method to extract MPs from the gastrovascular cavity of B. cangicum. The method consists of inducing egestion by pressing the anemone's central column with the aid of a stainless-steel instrument. The proposed technique was applied to 40 anemones sampled in the intertidal zone of a marine beach on the Brazilian Amazon coast. The recovered particles were characterized by Focal Plane Array Fourier Transform Infrared (FPA-FTIR) microscopy. The MP content obtained by the non-lethal method was then compared with that obtained by the lethal techniques applied previously at the same site. An additional 30 anemones from the same area were used in laboratory experiments to evaluate the method's effects on growth, survival, and predatory performance. The non-lethal method retrieved quantities, sizes and shapes of MPs similar to those recovered by dissection techniques. Furthermore, the exposure to the method had no significant effects on the organism's growth, survival and predatory performance. Therefore, the non-lethal method proved to be efficient in extracting MPs from the gastrovascular cavity of B. cangicum without causing deleterious effects. Thus, the proposed method enhances the species' potential as a biomonitor, as it easily allows numerous organisms to be adequately sampled without sacrificing them.

Capsule

The non-lethal method proved to be efficient in extracting MPs from the gastrovascular cavity of B. cangicum without causing deleterious effects.

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来源期刊

Marine pollution bulletin 环境科学-海洋与淡水生物学

CiteScore

10.20

自引率

15.50%

发文量

1077

审稿时长

68 days

期刊介绍： Marine Pollution Bulletin is concerned with the rational use of maritime and marine resources in estuaries, the seas and oceans, as well as with documenting marine pollution and introducing new forms of measurement and analysis. A wide range of topics are discussed as news, comment, reviews and research reports, not only on effluent disposal and pollution control, but also on the management, economic aspects and protection of the marine environment in general.