Using metagenomics to reveal the effects of zero-valent iron with different sizes on the mesophilic and thermophilic anaerobic digestion of sludge

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-02-18 DOI:10.1016/j.bej.2025.109688

Jianglin Chen , Wenqian Li , Lina Pang , Efthalia Chatzisymeon , Yuanyuan Lu , Ping Yang

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Abstract

This work investigated the mechanisms of anaerobic digestion (AD) of sludge to the addition of zero-valent iron (ZVI) at three particle sizes under mesophilic (MAD) and thermophilic (TAD) temperatures. Results showed that nano-ZVI, micron-ZVI, and iron scrap all enhanced the cumulative methane production in two AD processes. Besides, the highest cumulative methane yield (83.92 mL/g VS) was achieved in MAD with nano-ZVI, which possibly stimulated the hydrolysis and acidification processes in MAD. Moreover, metagenomic results revealed that the more abundant predominant methanogenic pathways and functional microbes like genera Candidatus_Microthrix and Methanothrix, and the enriched enzymes associated with hydrotrophic and methyl methanogenesis pathways, might be the underlying reasons for the best performance of MAD with nano-ZVI. This study provides knowledge underpinning the mechanisms for the deployment of ZVI in solid waste anaerobic digestion to effectively manage and treat waste and increase biomethane production.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.