Evaluation of male contraceptive efficacy of Caesalpinia pulcherrima (L.) sw. in human and rat: an ex-vivo study.

IF 2.1 4区 医学 Q3 ANDROLOGY
Pampa Lohar, Dibya Pal, Tanusree Mondal, Shibani Das, Puja Das, Debidas Ghosh
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Abstract

The study focused on the spermicidal and anti-androgenic effects of aqueous-ethanolic (60:40) extract of Caesalpinia pulcherrima leaves (AEECPL) in human and rat samples from the viewpoint of its contraceptive efficacy through ex-vivo study. Six fertile adult males were selected randomly for semen collection. Parallelly sperm samples were collected by epididymal washing from six rats. Testes, epididymis, and liver were dissected from rats. Biological samples were divided into control, 1, 2, and 4 mg/ml of AEECPL exposed groups. Relevant spermiological, steroidogenic enzymes, oxidative stress, and metabolic toxicity sensors were evaluated. All the spermiological sensors were decreased significantly in dose and duration-dependent manners, and the number of comet positive spermatozoa were increased in dose-dependent mode in AEECPL exposed groups against the control both in human and rat. Activities of Δ5,3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD in testis, kinetics of superoxide dismutase both in testis and epididymis were significantly decreased along with the elevation in the level of thiobarbituric acid reactive substances in AEECPL exposed groups. Activities of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, acid phosphatase, and alkaline phosphatase in above mentioned tissues showed no significant difference among the control and AEECPL exposed groups, indicating its non-toxic effects on reproductive and metabolic tissues. The results presenting the prominent contraceptive preventing potentiality of the said extract both in human and rat. The optimal effect was noted at 2 mg/ml dose. In-depth investigations are required through in-vivo studies on animal model to know the genomic mode of action for the execution of male contraceptive activity.

白腹剖宫产男性避孕效果评价。在人类和大鼠:离体研究。
通过离体研究,从避孕功效的角度,探讨了水乙醇(60:40)水乙醇提取物(AEECPL)在人体和大鼠体内的杀精和抗雄激素作用。随机选取6只有生育能力的成年雄性进行精液采集。同时对6只大鼠进行附睾洗涤,收集精子样本。解剖大鼠睾丸、附睾和肝脏。生物样品分为对照、1、2、4 mg/ml AEECPL暴露组。评估了相关的精子学、类固醇生成酶、氧化应激和代谢毒性传感器。与对照组相比,AEECPL暴露组的所有精子传感器均呈剂量依赖性和持续时间依赖性降低,彗星阳性精子数量呈剂量依赖性增加。随着硫代巴比托酸活性物质水平的升高,AEECPL暴露组大鼠睾丸中Δ5、3β-羟基类固醇脱氢酶(HSD)、17β-HSD活性以及睾丸和附睾超氧化物歧化酶动力学均显著降低。上述组织中谷草酰乙酸转氨酶、谷氨酸丙酮酸转氨酶、酸性磷酸酶和碱性磷酸酶的活性在对照组和AEECPL暴露组之间均无显著差异,说明AEECPL对生殖和代谢组织无毒性作用。结果表明,该提取物对人体和大鼠均有明显的避孕预防作用。剂量为2 mg/ml时效果最佳。需要通过动物模型的体内研究深入研究,以了解男性避孕活性执行的基因组作用模式。
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来源期刊
CiteScore
4.30
自引率
4.20%
发文量
27
审稿时长
>12 weeks
期刊介绍: Systems Biology in Reproductive Medicine, SBiRM, publishes Research Articles, Communications, Applications Notes that include protocols a Clinical Corner that includes case reports, Review Articles and Hypotheses and Letters to the Editor on human and animal reproduction. The journal will highlight the use of systems approaches including genomic, cellular, proteomic, metabolomic, bioinformatic, molecular, and biochemical, to address fundamental questions in reproductive biology, reproductive medicine, and translational research. The journal publishes research involving human and animal gametes, stem cells, developmental biology and toxicology, and clinical care in reproductive medicine. Specific areas of interest to the journal include: male factor infertility and germ cell biology, reproductive technologies (gamete micro-manipulation and cryopreservation, in vitro fertilization/embryo transfer (IVF/ET) and contraception. Research that is directed towards developing new or enhanced technologies for clinical medicine or scientific research in reproduction is of significant interest to the journal.
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