Antimicrobial photodynamic therapy using gold nanoparticles conjugated with 1,9-dimethyl-methylene blue for treating Staphylococcus skin infections.

IF 2.1 4区医学 Q3 ENGINEERING, BIOMEDICAL

Lasers in Medical Science Pub Date : 2025-02-19 DOI:10.1007/s10103-025-04367-1

Anildo Alves de Brito Júnior, Pedro Jorge Louro Crugeira, Wellington Luis Reis Costa, Andressa Vollono Barbosa, Susana Carla Pires Sampaio de Oliveira, Maria Cristina Teixeira Cangussu, Jean Nunes Dos Santos, Juliana Monteiro Azevedo, Antonio Luiz Barbosa Pinheiro

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Abstract

This study aims to assess the effectiveness of Antimicrobial Photodynamic Therapy (aPDT) using phenothiazine photosensitizer (PS) combined with gold nanoparticles (DMMB-AuNPs) in treating S. aureus-contaminated wounds on the dorsum of rats. Twelve Wistar rats were divided into four groups: Control, LED, DMMB-AuNPs, and DMMB-AuNPs + LED. DMMB-AuNPs were prepared using DMMB (300 ng/mL) and 5 nm diameter AuNPs (~ 5.5E + 13 particles/mL). The light source was a red LED (12 J/cm², λ630 ± 20 nm, CW, 125 mW, 192s). Following the procedures, samples were plated on an agar medium specific to S. aureus to count the colony-forming units (CFU/mL). Statistical analysis using unidirectional ANOVA and Tukey's test revealed that the aPDT group (DMMB-AuNPs + LED) exhibited a 99.999% (5 log reduction) microbial reduction with a significant statistical difference (p < 0.0001) compared to the Control group, demonstrating a bactericidal effect. The in vivo findings unequivocally demonstrate the effectiveness of DMMB-AuNPs-mediated aPDT as an alternative therapy for staphylococcal infections.

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利用1,9-二甲基亚甲基蓝偶联金纳米颗粒治疗葡萄球菌皮肤感染的抗菌光动力疗法。

本研究旨在评价吩噻嗪光敏剂（PS）联合金纳米粒子（DMMB-AuNPs）抗菌光动力疗法（aPDT）治疗大鼠背部金黄色葡萄球菌污染伤口的效果。12只Wistar大鼠分为4组：对照组、LED组、DMMB-AuNPs组和DMMB-AuNPs + LED组。用DMMB （300 ng/mL）和5 nm直径的AuNPs （~ 5.5E + 13粒/mL）制备DMMB-AuNPs。光源为红色LED （12 J/cm2, λ630±20 nm， CW, 125 mW, 192s）。随后，将样品涂于金黄色葡萄球菌专用琼脂培养基上计数菌落形成单位（CFU/mL）。采用单因素方差分析和Tukey检验的统计分析显示，aPDT组（DMMB-AuNPs + LED）的微生物数量减少了99.999%（5个对数减少），统计学差异显著(p

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来源期刊

Lasers in Medical Science 医学-工程：生物医学

CiteScore

4.50

自引率

4.80%

发文量

192

审稿时长

3-8 weeks

期刊介绍： Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.