Chan Xing, Jie Sun, Yaqian Liu, Jialin Zhou, Xue Bai, Jun Li, Haidong Zhao
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引用次数: 0
Abstract
Purpose: Secondary chronic lymphedema is a complication that seriously affects the quality of life of cancers survivors which is urgent to be studied. However, the current animal models generally have some defects such as short duration that affect the research process. To acquire an animal model which is easier to accomplish as well as higher success rate is the main goal of our experiment. Methods: The hind limb model of rats with secondary chronic lymphedema was established by near infrared fluorescence-guided lymphatic system destruction combined with high-fat diet innovation. The model was evaluated by limb circumference, volume, near-infrared fluorescence imaging of lymphatic vessels, and a series of histopathological staining. Results: The model was successfully established by operation combined with a high-fat diet and kept persistent effective lasting up to 16 weeks. Compared with the normal diet group, the high-fat group received a higher success rate (77.77% vs. 55.55%), a more obvious degree of limb swelling, and a more significant level of subcutaneous inflammatory factor infiltration at the edema site. Based on it, we kept research and found out that there was no significant difference in the process of subcutaneous fibrosis between the two groups. Conclusions: High-fat diet may improve secondary chronic lymphedema rat model success rate as well as effectiveness by changing the level of inflammatory infiltration in the site of lymphedema.
目的:继发性慢性淋巴水肿是一种严重影响癌症幸存者生活质量的并发症,亟待研究。然而,目前的动物模型普遍存在持续时间短等缺陷,影响了研究进程。获得一个更容易完成和成功率更高的动物模型是我们实验的主要目标。方法:采用近红外荧光引导淋巴系统破坏结合高脂饮食创新的方法,建立继发性慢性淋巴水肿大鼠后肢模型。通过四肢围度、体积、淋巴管近红外荧光成像及一系列组织病理学染色对模型进行评价。结果:手术联合高脂饮食成功建立模型,持续有效达16周。与正常饮食组相比,高脂组手术成功率更高(77.77% vs. 55.55%),肢体肿胀程度更明显,水肿部位皮下炎症因子浸润水平更显著。在此基础上,我们继续研究,发现两组间皮下纤维化过程无明显差异。结论:高脂饮食可能通过改变淋巴水肿部位的炎症浸润水平,提高继发性慢性淋巴水肿大鼠模型的成功率和疗效。
期刊介绍:
Lymphatic Research and Biology delivers the most current peer-reviewed advances and developments in lymphatic biology and pathology from the world’s leading biomedical investigators. The Journal provides original research from a broad range of investigative disciplines, including genetics, biochemistry and biophysics, cellular and molecular biology, physiology and pharmacology, anatomy, developmental biology, and pathology.
Lymphatic Research and Biology coverage includes:
-Vasculogenesis and angiogenesis
-Genetics of lymphatic disorders
-Human lymphatic disease, including lymphatic insufficiency and associated vascular anomalies
-Physiology of intestinal fluid and protein balance
-Immunosurveillance and immune cell trafficking
-Tumor biology and metastasis
-Pharmacology
-Lymphatic imaging
-Endothelial and smooth muscle cell biology
-Inflammation, infection, and autoimmune disease