Study on the mechanism of action of berberine combined with Jianpi Yishen Huazhuo formulation in treating obese polycystic ovary syndrome by activating PI3K/AKT signaling pathway.

IF 2 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Gynecological Endocrinology Pub Date : 2025-12-01 Epub Date: 2025-02-18 DOI:10.1080/09513590.2025.2462068
Xiaojing Zhang, Dejiang Ji, Yan Zhang, Cuizhong Du, Lijun Liang, Aqsa Ahmad, Yahong Feng, Gaxi Ye
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引用次数: 0

Abstract

Objectives: This study aims to investigate the effects and potential mechanisms of berberine in conjunction with Jianpi Yishen Huazhuo formulation (JPYSHZF) on obese rats that serve as a model for polycystic ovary syndrome (PCOS).

Methods: Letrozole combined with high-fat diet (HFD) was used to establish an overweight PCOS rat model. After successful modeling, each intervention group was monitored for 28 d. An oral glucose tolerance test (OGTT) is performed to assess glucose metabolism. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of sex hormones and serum levels of gastrointestinal hormones in rats. Biochemical analyzers were used to assess blood lipid levels. The protein expression levels of p38, PI3K, GLUT4, and AKT in ovarian tissue were demonstrated using Western Blotting (WB). Real-time fluorescence quantitative PCR (RT-qPCR) was used to measure the mRNA expression levels of p38, PI3K, GLUT4 and AKT in the same tissue. The morphological changes of ovarian tissue were observed using Hematoxylin-eosin (HE).

Results: Treatment with berberine in conjunction with JPYSHZF has been shown to reduce serum testosterone T and luteinizing hormone (LH) levels while increasing serum follicle-stimulating hormone (FSH) and E2 levels. This combination therapy also decreases the LH/FSH ratio and ameliorates polycystic ovary-like pathological changes in the ovaries of rats with PCOS. Additionally, this treatment decreases serum TC, TG, and LDL-c levels while increasing HDL-c levels. It also reduces levels of GLU and Ghrelin while enhancing levels of CCK, PYY, and GLP-1. Furthermore, the relative 6 of PI3K and AKT proteins, as well as the mRNA levels of PI3K, GLUT4, and AKT, were found to be increased.

Conclusions: Berberine combined with JPYSHZF can improve the sex hormone levels, ovarian function, glucose and lipid metabolism levels, and gastrointestinal hormone levels in obese PCOS rats by activating the PI3K/AKT signaling pathway, thereby playing a role in treating obese PCOS.

小檗碱联合健脾益肾化浊方激活PI3K/AKT信号通路治疗肥胖型多囊卵巢综合征的作用机制研究
目的:探讨小檗碱联合健脾益肾化浊方(JPYSHZF)对肥胖大鼠多囊卵巢综合征(PCOS)模型的影响及其机制。方法:采用来曲唑联合高脂饮食(HFD)建立超重PCOS大鼠模型。造模成功后,各干预组监测28 d。口服糖耐量试验(OGTT)评估糖代谢。采用酶联免疫吸附法(ELISA)测定大鼠性激素水平和血清胃肠激素水平。使用生化分析仪评估血脂水平。Western Blotting (WB)检测卵巢组织中p38、PI3K、GLUT4、AKT蛋白的表达水平。采用实时荧光定量PCR (RT-qPCR)检测p38、PI3K、GLUT4、AKT在同一组织中的mRNA表达水平。采用苏木精-伊红(HE)法观察卵巢组织形态学变化。结果:小檗碱联合JPYSHZF治疗可降低血清睾酮T和黄体生成素(LH)水平,同时增加血清促卵泡激素(FSH)和E2水平。该联合治疗还可降低多囊卵巢综合征大鼠卵巢的LH/FSH比值,改善多囊卵巢样病理改变。此外,这种治疗降低血清TC、TG和LDL-c水平,同时增加HDL-c水平。它还能降低GLU和Ghrelin的水平,同时提高CCK、PYY和GLP-1的水平。此外,PI3K和AKT蛋白的相对6以及PI3K、GLUT4和AKT的mRNA水平均升高。结论:小檗碱联合JPYSHZF可通过激活PI3K/AKT信号通路,改善肥胖型PCOS大鼠性激素水平、卵巢功能、糖脂代谢水平及胃肠道激素水平,从而起到治疗肥胖型PCOS的作用。
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来源期刊
Gynecological Endocrinology
Gynecological Endocrinology 医学-妇产科学
CiteScore
4.40
自引率
5.00%
发文量
137
审稿时长
3-6 weeks
期刊介绍: Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction
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