A Novel Hepcidin Isoform Jd-Hep from the Sin Croaker Johnius dussumieri (Cuvier, 1830): Recombinant Expression and Insights into the Antibacterial Property and Modes of Action

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
M. V. Anju, K. Archana, S. Muhammed Musthafa, V. V. Anooja, P. P. Athira, S. Neelima, M. Dhaneesha, T. P. Sajeevan, I. S. Bright Singh, Rosamma Philip
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Abstract

Hepcidin is a cysteine-rich antimicrobial peptide that plays an important role in fish immunity. In the current study, we report a novel isoform of hepcidin (Jd-Hep) from Sin croaker, Johnius dussumieri, with an open reading frame (ORF) of 258 nucleotide bases that encodes 85 amino acids containing a signal peptide (24 amino acids), a prodomain (35 amino acids) and a biologically active mature peptide (26 amino acids). Phylogenetic tree analysis showed that J. dussumieri hepcidin belonged to the HAMP2 cluster of hepcidin. The tissue distribution showed that the expression of hepcidin was highest in the liver in wild-caught J. dussumieri. The mature peptide mJd-Hep was recombinantly expressed in a prokaryotic host, E. coli Rosetta-gami™B (DE3) pLysS cells, and the peptide was isolated and purified. The recombinant peptide, rJd-Hep, exhibited notable antibacterial activity against aquatic pathogens such as Aeromonas hydrophila, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio proteolyticus, and Vibrio fluvialis. The mode of action of the peptide was proven to be membrane-based (pore formation and depolarization). The rJd-Hep was found to be non-hemolytic to hRBCs and non-cytotoxic to the mammalian cell line. The peptide showed 85% growth inhibition of cancer cell line, MCF-7. These findings expand our knowledge of the potential application of hepcidin in aquaculture as a therapeutic agent.

一种新的Hepcidin异构体j - hep来自sincroaker Johnius dussumieri(居维叶,1830):重组表达和对抗菌特性和作用方式的见解
Hepcidin是一种富含半胱氨酸的抗菌肽,在鱼类免疫中起重要作用。在目前的研究中,我们报道了一种来自sincroaker, Johnius dussumieri的hepcidin (j - hep)的新异构体,具有258个核苷酸碱基的开放阅读框(ORF),编码85个氨基酸,其中包含一个信号肽(24个氨基酸),一个原结构域(35个氨基酸)和一个生物活性成熟肽(26个氨基酸)。系统进化树分析表明,dussumieri hepcidin属于hepcidin的HAMP2簇。组织分布表明,hepcidin在野生家蝇肝脏的表达量最高。在原核宿主大肠杆菌Rosetta-gami™B (DE3) pLysS细胞中重组表达成熟肽mJd-Hep,并分离纯化该肽。重组肽rJd-Hep对嗜水气单胞菌、副溶血性弧菌、哈维弧菌、溶藻弧菌、溶蛋白弧菌和流感弧菌等水生病原菌均有显著的抑菌活性。肽的作用模式被证明是基于膜的(孔形成和去极化)。发现rdd - hep对hrbc无溶血作用,对哺乳动物细胞系无细胞毒性。该肽对癌细胞MCF-7有85%的抑制作用。这些发现扩大了我们对hepcidin作为治疗剂在水产养殖中的潜在应用的认识。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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