Visualization of the spatial distribution of the heartwood constituents of Dalbergia odorifera

IF 3.1 2区农林科学 Q1 FORESTRY

Wood Science and Technology Pub Date : 2025-02-19 DOI:10.1007/s00226-025-01638-6

Xiaodie Lu, Yunlin Fu, Penglian Wei, Mengji Qiao

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Abstract

The heartwood of Dalbergia odorifera T. Chen (D. odorifera) is esteemed for its high flavonoid content. This study aimed to explore the spatial distribution of heartwood components in the xylem, analyze their accumulation patterns, and further discuss the synthesis and migration pathways of these components. To achieve this, DESI-MSI technology was employed, leveraging the specific interaction between flavonoids and the naturally derived compound Naturstoff A (diphenylboronic acid 2-aminoethyl ester), alongside Raman microscopy techniques to elucidate the in situ distribution of heartwood extracts in the xylem. Additionally, combined with microscopic observations of the pits, the study investigated the accumulation and migration pathways of heartwood components in D. odorifera. The findings indicated that the total flavonoid content increased from sapwood to heartwood and subsequently decreased, with the difference in fluorescence intensity before and after extraction displaying a similar trend. The distribution pattern of various flavonoids in the xylem was as follows: heartwood > transition zone > sapwood, and this distribution was uneven among different tissues within the same region. Notably, within the same region, the flavonoid accumulation in axial parenchyma cells was greater than that in ray parenchyma cells. Moreover, the cell corners were identified as the main sites of deposition for heartwood extracts. This research underscores the distribution patterns of heartwood constituents within the xylem, providing a scientific foundation for the conservation, enhancement, and utilization of D. odorifera heartwood resources.

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降香黄檀心材成分空间分布的可视化研究

黄檀（Dalbergia odorifera T. Chen）的心材因其高黄酮含量而备受推崇。本研究旨在探索心材成分在木质部的空间分布，分析其积累模式，并进一步探讨这些成分的合成和迁移途径。为了实现这一目标，采用了DESI-MSI技术，利用黄酮类化合物与天然衍生化合物Naturstoff A（二苯硼酸2-氨基乙酯）之间的特异性相互作用，结合拉曼显微镜技术来阐明木质部中心材提取物的原位分布。此外，结合果核的显微观察，研究了降香草心材成分在果核中的积累和迁移途径。结果表明，总黄酮含量从边材到心材先升高后降低，提取前后的荧光强度差异也呈现出相似的趋势。木质部中各类黄酮类化合物的分布规律为：心材+过渡区+边材，同一区域内不同组织间的分布不均匀。值得注意的是，在同一区域内，轴向薄壁细胞的类黄酮积累量大于射线薄壁细胞。细胞角是心材提取物的主要沉积部位。本研究揭示了香薷木质部内心材成分的分布规律，为香薷心材资源的保护、增值和利用提供了科学依据。

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来源期刊

Wood Science and Technology 工程技术-材料科学：纸与木材

CiteScore

5.90

自引率

5.90%

发文量

审稿时长

3 months

期刊介绍： Wood Science and Technology publishes original scientific research results and review papers covering the entire field of wood material science, wood components and wood based products. Subjects are wood biology and wood quality, wood physics and physical technologies, wood chemistry and chemical technologies. Latest advances in areas such as cell wall and wood formation; structural and chemical composition of wood and wood composites and their property relations; physical, mechanical and chemical characterization and relevant methodological developments, and microbiological degradation of wood and wood based products are reported. Topics related to wood technology include machining, gluing, and finishing, composite technology, wood modification, wood mechanics, creep and rheology, and the conversion of wood into pulp and biorefinery products.