Genetic analysis of Vibrio alginolyticus challenged by Scutellaria baicalensis reveals the mechanism of virulence genes

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-02-13 DOI:10.1016/j.genrep.2025.102159

Genhuang Xu , Xiaojin Xu , Rui Lai , Zhixing Zhang , Yongqi Lai , Kejin Dai , Xin Yi , Minglan Guo

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引用次数: 0

Abstract

Scutellaria baicalensis can be used to treat bacterial infections caused by Vibrio alginolyticus, with marked inhibition at 1200 μg/mL. S. baicalensis significantly impacts the motility and biofilm formation capabilities of V. alginolyticus. V. alginolyticus exhibits a discerning sensitivity profile to antibiotics, demonstrating resistance against beta-lactam antibiotics such as penicillins and oxacillins, while showing susceptibility to a broader spectrum of antibiotic classes, including tetracyclines, aminoglycosides, and others. Under S. baicalensis stress, flagellar biogenesis genes (fliP, fliQ, fliR) exhibit concentration-dependent expression changes, declining at higher concentrations. The expression profiles of rpoD and rpoS initially show an upregulation but subsequently decline under higher stress levels. Conversely, glpD presents a continuously declining expression pattern regardless of the intensity of the stress condition. After treating V. alginolyticus with 8 mg/mL of S. baicalensis for 2 h and 4 h, transcriptome analysis identified 2350 differentially expressed genes (DEGs). These DEGs are enriched in 29 distinct secondary Gene Ontology (GO) categories, among which metabolic processes are the most significantly represented. KEGG analysis indicated that amino acid metabolism pathways and two-component system pathways play important roles. Chemotaxis pathways related to bacterial movement and response to external stimuli have also been notably affected.

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黄芩攻毒溶藻弧菌的遗传分析揭示了毒力基因的作用机制

黄芩可用于治疗溶藻弧菌引起的细菌感染，在1200 μg/mL时具有明显的抑制作用。黄芩对溶藻弧菌的活力和生物膜形成能力有显著影响。溶藻弧菌对抗生素表现出明显的敏感性，对-内酰胺类抗生素（如青霉素类和恶西林类）具有耐药性，同时对更广泛的抗生素类别（包括四环素类、氨基糖苷类和其他抗生素）具有敏感性。在黄芩胁迫下，鞭毛发生基因（fliP、fliQ、fliR）表现出浓度依赖性表达变化，浓度越高表达量越低。rpoD和rpoS的表达谱在较高的应激水平下呈现先上调后下降的趋势。相反，无论应激条件的强度如何，glpD都呈现出持续下降的表达模式。8 mg/mL黄芩苷处理溶藻弧菌2 h和4 h后，转录组分析鉴定出2350个差异表达基因（deg）。这些deg在29个不同的次级基因本体（GO）类别中丰富，其中代谢过程的代表性最显著。KEGG分析表明，氨基酸代谢途径和双组分系统途径发挥了重要作用。与细菌运动和对外部刺激的反应相关的趋化途径也受到了显著影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.