Berberine loaded glyceryl monooleate nanoparticles exhibited potent intrinsic anticancer activity against pancreatic cancer therapy: In vitro and in silico studies

Md․ Harun Al Rashid , Sweta Mishra , Saswati Pattnaik , Chandana Mohanty
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Abstract

Berberine (BER) has emerged as a promising anticancer medication that can induce antiproliferative effects in several cancers, including pancreatic cancer. However, its clinical application is limited by poor solubility and bioavailability. To overcome these challenges, BER-loaded glyceryl monooleate nanoparticles (BER-GNPs) were developed to enhance its delivery and therapeutic efficacy. The synthesized BER-GNPs were characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR), which confirmed their favorable size, morphology, and stability. In vitro studies demonstrated that BER-GNPs significantly increased cytotoxicity and inhibited cell migration and invasion in pancreatic cancer cells (PANC-1) compared to native BER. Furthermore, in silico studies suggested that BER may inhibit tumor cell proliferation by targeting the AKT signaling pathway. This was further validated through western blot analysis which confirmed that BER-GNPs significantly suppressed AKT phosphorylation and activated the intrinsic apoptotic pathway by increasing the Bax/Bcl-2 ratio, indicating enhanced pro-apoptotic activity.

Abstract Image

含有小檗碱的单油酸甘油纳米颗粒对胰腺癌治疗表现出强大的内在抗癌活性:体外和硅研究
小檗碱(Berberine, BER)已成为一种很有前景的抗癌药物,可以诱导包括胰腺癌在内的几种癌症的抗增殖作用。但其溶解度和生物利用度较差,限制了其临床应用。为了克服这些挑战,ber负载的单油酸甘油酯纳米颗粒(BER-GNPs)被开发出来,以增强其递送和治疗效果。利用动态光散射(DLS)、扫描电镜(SEM)和傅里叶变换红外光谱(FTIR)对合成的BER-GNPs进行了表征,证实了其良好的尺寸、形貌和稳定性。体外研究表明,与天然BER相比,BER- gnps显著增加胰腺癌细胞毒性,抑制细胞迁移和侵袭(PANC-1)。此外,计算机研究表明,BER可能通过靶向AKT信号通路抑制肿瘤细胞增殖。western blot分析进一步验证了这一点,证实了BER-GNPs通过增加Bax/Bcl-2比值,显著抑制AKT磷酸化,激活内在凋亡通路,促凋亡活性增强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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