Overexpression of Cucurbita pepo CpVQ27 increased susceptibility of tobacco to powdery mildew

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-02-17 DOI:10.1016/j.pmpp.2025.102614

Shi-heng Li, Ping Wang

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引用次数: 0

Abstract

Powdery mildew (PM) is the primary disease affecting Cucurbita pepo cultivation, and one of the significant factors influencing its yield. The VQ protein is involved in a diverse range of signal transduction pathways, exerting influences on plant growth and development, while also playing a pivotal role in response to both biotic and abiotic stresses. This study investigates the response of CpVQ27 (XM_023689058.1) gene in Cucurbita pepo to powdery mildew infection. Its function in tobacco powdery mildew was examined through genetic transformation. Compared to the wild-type (WT), CpVQ27-overexpression tobacco exacerbated the powdery mildew symptoms. Antioxidant enzyme activities decreased, while reactive oxygen species (ROS) and malondialdehyde (MDA) levels increased. Powdery mildew mycelium growth rate and biomass also increased. The disease resistance-related genes expression decreased in CpVQ27-overexpression tobacco. Overexpression of CpVQ27 enhances the susceptibility of tobacco to powdery mildew.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.