Integrating manual preprocessing with automated feature extraction for improved rodent seizure classification

Abstract

Hypothesis/Objective

Rodent models of epilepsy can help with the search for more effective drug candidates or neuromodulatory therapies. Yet, preclinical screening of candidate options for anti-epileptic drugs (AED) using rodent models may require hours of video monitoring. Data processing is also time-consuming, subjective, and error-prone. This study aims to develop an AI-enabled quantitative analysis of rodent behavior, including epilepsy stage classification.

Approach/Method

We leveraged deep learning and computer vision techniques to develop a semi-automatic pipeline and framework for animal seizure detection and recognition, which requires manual preprocessing of the dataset. Our hybrid approach combines model-based and data-driven methods but is dependent on manually preprocessed and segmented video clips to facilitate the automatic classification of epilepsy stages.

Results

We collected two datasets comprising rat skeleton keypoints and seizure behavior videos in the lab. The proposed method, PoseC3D, for rat seizure stage classification of the collected database achieved an accuracy between 64.7–90.3 % when tested on four different seizure phenotypes using the Racine classification scale.

Conclusions

This study demonstrates the feasibility of video-based seizure stage detection and classification for rodent models of temporal lobe seizures using a semi-automatic pipeline that requires manual preprocessing of data. However, our method is not capable of fully automated seizure detection and has not been tested on unseen animals, which limits its generalizability and applicability for broader use. Despite these limitations, the approach underscores our ability to undertake quantitative analysis of rodent behavior, which can also support other studies of animal behavior involving motor functions and future considerations for non-motor symptomology such as mood disorders.