Scalable production of muscle and adipose cell-laden microtissues using edible macroporous microcarriers for 3D printing of cultured fish fillets

IF 14.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Xuan Zhou, Hongwei Zheng, Yanchi Wu, Haowen Yin, Xiangzhao Mao, Ningyang Li, Huarong Guo, Yaoguang Chang, Xiaoming Jiang, Qinghui Ai, Changhu Xue
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Abstract

Cellular agriculture is a novel platform for addressing the issues of protein scarcity, environmental pressures, and food safety. However, expanding seed cells at a large scale remains a prerequisite for achieving industrial cultured meat production. We here propose the production of large-pore-sized edible porous microcarriers (EPMs) by using NaCl to precisely control ice crystal growth during cryogenic crosslinking. Muscle satellite cells (SCs) and adipose-derived stem cells (ASCs) from large yellow croakers exhibit remarkable adhesion, proliferation, and differentiation on gelatin-based EPMs. Following consecutive expansion, SCs and ASCs densities reach 6.25 × 105 and 5.77 × 105 cells/mL, leading to a 499-fold and 461-fold increase in cell numbers, respectively. Subsequently, the mature microtissues are incorporated into a bioink, thereby enabling successful three-dimensional printing of cultured fish fillets with sensory properties similar to native fish fillets. EPM-based cell expansion and food structuring techniques are promising in facilitating large-scale cultured fish meat production.

Abstract Image

使用可食用的大孔微载体用于3D打印养殖鱼片的肌肉和脂肪细胞负载微组织的可扩展生产
细胞农业是解决蛋白质短缺、环境压力和食品安全问题的新平台。然而,大规模扩大种子细胞仍然是实现工业化养殖肉类生产的先决条件。本文提出利用NaCl在低温交联过程中精确控制冰晶生长,制备大孔径可食用多孔微载体(epm)。来自大黄鱼的肌肉卫星细胞(SCs)和脂肪来源干细胞(ASCs)在明胶基epm上表现出显著的粘附、增殖和分化。连续扩增后,SCs和ASCs密度分别达到6.25 × 105和5.77 × 105个细胞/mL,细胞数量分别增加499倍和461倍。随后,将成熟的微组织纳入生物墨水中,从而成功地三维打印出具有与本地鱼片相似感官特性的养殖鱼片。基于epm的细胞扩增和食品结构技术在促进大规模养殖鱼肉生产方面具有前景。
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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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