Genetic identification and characterization of two novel loci for grain arsenic concentration in tetraploid wheat at various field environments

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2025-02-19 DOI:10.1007/s11104-025-07303-y

Zhaoyong Zeng, Dandan Ou, Li Yin, Yueyi Wu, Yuanfeng Huo, Yinggang Xu, Huaping Tang, Shu Yuan, Xuesong Gao, Yusheng Qin, Jian Ma, Guangdeng Chen

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引用次数: 0

Abstract

Aims

Arsenic (As) is a highly toxic metalloid that can accumulate in wheat, posing significant human health risks. However, the genetic basis underlying As accumulation in wheat grains remains largely unexplored.

Methods

This study utilized a recombinant inbred line (RIL) population derived from an endemic tetraploid wheat variety and a wild emmer accession. Phenotypic data were collected from three field environments and a pot experiment with three As levels.

Results

Seven quantitative trait loci (QTL) associated with grain As concentration (GrAsc) were identified. Among these, two major QTL—QGrAsc.sau-AM-1A and QGrAsc.sau-AM-4A— were located on chromosomes 1A and 4A, respectively, and were detected in over four environments. These loci, which explained 7.96% to 12.51% and 10.20% to 21.45% of phenotypic variance, respectively, and were successfully validated using Kompetitive Allele-Specific PCR (KASP) markers in a natural population. Additionally, four wheat varieties with low As concentrations were screened using KASP markers. Comparisons with previous studies suggest that these two major QTL are likely novel. Furthermore, the effects of QGrAsc.sau-AM-1A and QGrAsc.sau-AM-4A on GrAsc were analyzed. Candidate genes related to As uptake and transport were predicted to be associated with these loci. Correlation analysis between GrAsc and nine agronomic traits revealed a significant negative correlation with thousand kernel weight (TKW). Additionally, QGrAsc.sau-AM-1A was found to significantly increase spikelet number per spike, while QGrAsc.sau-AM-4A was associated with increased spike density. Overall, these results suggest that QGrAsc.sau-AM-1A and QGrAsc.sau-AM-4A are promising loci for further fine mapping and molecular breeding aimed at reducing As accumulation in wheat.

Conclusions

Two novel, major QTL—QGrAsc.sau-AM-1A and QGrAsc.sau-AM-4A— were identified for grain arsenic concentration. Their effects were validated in a natural wheat population, offering the potential for marker-assisted selection (MAS) and molecular breeding.

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.