Can Oral Swabs Be Used to Diagnose Nontuberculous Mycobacterial Cervicofacial Lymphadenitis?

IF 2.2 3区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Laryngoscope Pub Date : 2025-02-18 DOI:10.1002/lary.32062

Samuel H Willemse, Luc H E Karssemakers, Marjolijn A E M Oomens, Willem H Schreuder, Jean-Pierre T F Ho, Marion Kolader, Robin van Houdt, Martijn van der Kuip, Gina R Buijsers, Jan de Lange, Jerome A Lindeboom

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引用次数: 0

Abstract

Objective: We investigated the presence of mycobacteria in the oral cavity and oropharynx of children with nontuberculous mycobacterial (NTM) cervicofacial lymphadenitis compared to negative controls. Our aim was to evaluate whether oral cavity and oropharyngeal swab sampling could serve as a minimally invasive alternative to diagnostic sampling of excised lymph node tissue or punctate.

Methods: Children who underwent surgery under general anesthesia due to a strong clinical suspicion of NTM cervicofacial lymphadenitis were enrolled in this multicenter cross-sectional study. The control group comprised children undergoing surgery under general anesthesia for reasons other than lymphadenopathy. The obtained oropharyngeal, dorsal tongue, and gingival swabs were subjected to mycobacterial polymerase chain reaction (PCR) analysis.

Results: A total of 67 subjects were enrolled in this study, 31 of whom underwent surgery due to strong clinical suspicion of NTM cervicofacial lymphadenitis. Positive genus and Mycobacterium avium specific PCR results were found in one patient from the control group, and in none of the patients with (suspected) NTM cervicofacial lymphadenitis.

Conclusion: Our in-house routine diagnostic PCR assay on oral and oropharyngeal swabs was not suitable as a minimally invasive alternative to diagnostic sampling of excised lymph node material or punctate. It remains unclear whether the oral cavity and oropharynx might be a portal of entry for mycobacteria in NTM cervicofacial lymphadenitis.

Level of evidence: 2:

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来源期刊

Laryngoscope 医学-耳鼻喉科学

CiteScore

6.50

自引率

7.70%

发文量

500

审稿时长

2-4 weeks

期刊介绍： The Laryngoscope has been the leading source of information on advances in the diagnosis and treatment of head and neck disorders since 1890. The Laryngoscope is the first choice among otolaryngologists for publication of their important findings and techniques. Each monthly issue of The Laryngoscope features peer-reviewed medical, clinical, and research contributions in general otolaryngology, allergy/rhinology, otology/neurotology, laryngology/bronchoesophagology, head and neck surgery, sleep medicine, pediatric otolaryngology, facial plastics and reconstructive surgery, oncology, and communicative disorders. Contributions include papers and posters presented at the Annual and Section Meetings of the Triological Society, as well as independent papers, "How I Do It", "Triological Best Practice" articles, and contemporary reviews. Theses authored by the Triological Society’s new Fellows as well as papers presented at meetings of the American Laryngological Association are published in The Laryngoscope. • Broncho-esophagology • Communicative disorders • Head and neck surgery • Plastic and reconstructive facial surgery • Oncology • Speech and hearing defects