[Impact of Different Vegetation Restoration Types on Soil Microbial Community Structure in the Restoration Area of Quarries in Northern Hebei Province].

Abstract

Soil microorganisms have a strong influence on the soil environment of mining sites and the effectiveness of vegetation restoration. To investigate the response of soil microbial diversity in quarries in northern Jibei, China, to different types of vegetation restoration, we considered the common restoration vegetation in the area （YS, SJ, MX, MH, and CH） as the object of study and analyzed inter-root soil physicochemical factors and vegetation microbial community structure using the techniques of soil nutrient determination, high-throughput sequencing, and other methods. The results showed that： ① The type of vegetation restoration had a significant effect on the inter-root soil environment, and the alkaline dissolved nitrogen content of the inter-root soil of sea buckthorn and oil pine； the organic matter content of the soil of cotton acacia； and the microbial carbon content of lucerne, cotton acacia, and acacia were significantly higher than that of the control group （P<0.05）. ② The community composition and diversity of bacteria and fungi differed significantly among the different vegetation types. Proteobacteria and Ascomycota were the main dominant populations of bacteria and fungi, respectively. The relative abundance of Anabaena was higher than that of other plants in oil pine and sea buckthorn, and the ACE and Chao1 indices of lucerne and acacia differed significantly （P<0.05）, and the intergroup differences between different treatment groups were obvious （stress<0.1）. ③ The microbial communities of different restoration types were significantly correlated with soil factors, and the structure of bacterial and fungal communities showed highly significant correlations with SOM, TN, and MBC （P<0.01）. Among the bacterial communities, Acidobacteriota was positively correlated with microbial nitrogen content, alkaline dissolved nitrogen content, etc. Among the fungal communities； Olpidiomycota was positively correlated with TK and pH； the individual effects of nutrient factors were greatest in the bacterial phylum-level communities, and the synergistic effects of nutrient factors and enzyme activity factors were greatest in the fungal phylum-level communities. The conclusions of the study have theoretical implications for subsequent ecological restoration work in the mining area.