A Modified Intrabony Defect Model of Periodontitis in Rat.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-02-17 DOI:10.1111/odi.15284

Pei Cao, Chen-Hao Yu, Yuan-Qing Liu, Liang Chen, Qing-Xian Luan, Jia Liu

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引用次数: 0

Abstract

Objective: The aim of this study was to compare the radiographic depth of the periodontal bone defect, as well as clinical and histological outcomes, between the proposed ligated defect model and the current non-ligated defect model, which often heals spontaneously and fails to replicate the complexities of periodontitis.

Methods: The proposed model combined osteotomy to create controlled periodontal bone defects (depth of 1.5 mm) and ligature placement around the cervical region of the maxillary first molar to induce inflammation. Micro-CT analysis, clinical examinations, histological evaluations, and quantitative PCR were conducted to assess defect morphology, healing, and inflammatory marker expression over 6 weeks, compared with the non-ligated defect group, sham surgery group, and control.

Results: Bone defect in the ligated defect group remained unhealed after surgery (1.525 ± 0.068 mm in depth at 2 weeks and 1.543 ± 0.082 mm at 6 weeks), with inflammatory cell infiltration and elevated expression of IL-1β, IL-6, and TNF-α, contrasting with observed healing from 1.328 ± 0.075 mm (2 weeks) to 0.593 ± 0.118 mm (6 weeks) in the non-ligated defect group.

Conclusions: This model not only simulates the pathological features of periodontitis but also provides a reliable platform for testing innovative regenerative strategies and could lead to the development of specialized models for other periodontal conditions.

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改良的大鼠牙周炎骨内缺损模型。

目的：本研究的目的是比较所提出的结扎缺损模型和目前的非结扎缺损模型的牙周骨缺损的x线深度、临床和组织学结果，后者通常是自发愈合的，不能复制牙周炎的复杂性。方法：采用截骨术制造可控牙周骨缺损（深度1.5 mm），并在上颌第一磨牙颈区周围结扎诱导炎症。与未结扎缺损组、假手术组和对照组比较，通过显微ct分析、临床检查、组织学评估和定量PCR评估缺损形态、愈合情况和炎症标志物表达。结果：结扎缺损组骨缺损术后未愈合（2周深度1.525±0.068 mm， 6周深度1.543±0.082 mm），伴有炎性细胞浸润，IL-1β、IL-6、TNF-α表达升高，而未结扎缺损组骨缺损愈合为1.328±0.075 mm（2周）~ 0.593±0.118 mm（6周）。结论：该模型不仅模拟了牙周炎的病理特征，而且为测试创新的再生策略提供了可靠的平台，并可能导致其他牙周疾病的专门模型的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.