A Modified Intrabony Defect Model of Periodontitis in Rat.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-02-17 DOI:10.1111/odi.15284

Pei Cao, Chen-Hao Yu, Yuan-Qing Liu, Liang Chen, Qing-Xian Luan, Jia Liu

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引用次数: 0

Abstract

Objective: The aim of this study was to compare the radiographic depth of the periodontal bone defect, as well as clinical and histological outcomes, between the proposed ligated defect model and the current non-ligated defect model, which often heals spontaneously and fails to replicate the complexities of periodontitis.

Methods: The proposed model combined osteotomy to create controlled periodontal bone defects (depth of 1.5 mm) and ligature placement around the cervical region of the maxillary first molar to induce inflammation. Micro-CT analysis, clinical examinations, histological evaluations, and quantitative PCR were conducted to assess defect morphology, healing, and inflammatory marker expression over 6 weeks, compared with the non-ligated defect group, sham surgery group, and control.

Results: Bone defect in the ligated defect group remained unhealed after surgery (1.525 ± 0.068 mm in depth at 2 weeks and 1.543 ± 0.082 mm at 6 weeks), with inflammatory cell infiltration and elevated expression of IL-1β, IL-6, and TNF-α, contrasting with observed healing from 1.328 ± 0.075 mm (2 weeks) to 0.593 ± 0.118 mm (6 weeks) in the non-ligated defect group.

Conclusions: This model not only simulates the pathological features of periodontitis but also provides a reliable platform for testing innovative regenerative strategies and could lead to the development of specialized models for other periodontal conditions.

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.