Molecular characterization and subtype analysis of Blastocystis sp. in captive wildlife in Henan, China.

IF 2.3 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2025-01-01 Epub Date: 2025-02-17 DOI:10.1051/parasite/2025006

Zhaohui Cui, Xiyao Huang, Sulan Zhang, Kaifang Li, Aili Zhang, Qichen Li, Yutong Zhang, Junqiang Li, Meng Qi

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引用次数: 0

Abstract

Blastocystis sp. is a zoonotic intestinal protozoan that is ubiquitous globally, residing in the gastrointestinal tracts of both humans and various animals. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was employed to investigate the prevalence and subtypes of Blastocystis spp. in 204 fresh fecal samples collected from 20 captive wildlife species from a bird park in Henan Province, Central China. Overall, Blastocystis was present in 13.73% (28 out of 204) of the samples and 25% (5 out of 20) of the species. A total of four zoonotic subtypes of Blastocystis sp. were found: ST1, ST3, ST5, and ST27, with the latter being the most prevalent, accounting for 35.71% (10 out of 28) of the 5 species positive for Blastocystis sp. To the best of our knowledge, this is the first report of Blastocystis ST27 in birds in China, namely bar-headed goose (Anser indicus) and peafowl (Pavo muticus). The data suggest that captive wildlife, particularly those in bird parks, may frequently be infected with this zoonotic pathogen. Consequently, these animals may serve as potential reservoirs for zoonotic infections in humans.

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河南圈养野生动物囊虫的分子特征及亚型分析。

囊虫是一种人畜共患的肠道原生动物，在全球范围内普遍存在，存在于人类和各种动物的胃肠道中。本研究采用基于SSU rRNA基因的pcr测序工具，对河南省某鸟类公园20种圈养野生动物204份新鲜粪便中囊虫属的流行情况和亚型进行了调查。总体而言，204份样本中有28份（13.73%）存在囊虫，20份样本中有5份（25%）存在囊虫。共检出囊虫属4种人畜共患亚型：ST1、ST3、ST5和ST27，其中以ST27最常见，在5种囊虫属阳性种中占35.71%（28种中有10种）。据我们所知，这是中国首次报道囊虫属ST27感染禽类，即斑头雁和孔雀。数据表明，圈养野生动物，特别是鸟类公园的野生动物，可能经常感染这种人畜共患病原体。因此，这些动物可能成为人类人畜共患感染的潜在宿主。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.