Detection of an Alphacoronavirus in a Brazilian Bat (Molossus sp.).

IF 2.1 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Molecular Evolution Pub Date : 2025-04-01 Epub Date: 2025-02-17 DOI:10.1007/s00239-025-10236-w

C L Molina, M M Magalhães, A C Rodrigues, S A Taniwaki, S O de Souza Silva, G A König, P E Brandão

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引用次数: 0

Abstract

Due to the COVID-19 pandemic and the uncertainty about aspects of its origin, in recent years there has been an increased interest in investigating coronaviruses in wild animals. Bats are hosts of the greatest diversity of coronaviruses to date, including the ancestors of viruses that have caused outbreaks in humans. Although in Brazil, information on coronaviruses in bats has expanded, still they remain unrepresentative. To help shed some light on this matter, we collected 175 samples from bats of different species from two Brazilian states. Here, we report the previously unknown presence of an alphacoronavirus in a bat (Molossus sp.) from Ceará. The phylogenetic analysis showed close relationships with alphacoronaviruses from Brazil and Argentina, but it was not possible to determine the subgenus or species of this virus using RNA-dependent RNA-polymerase (RdRp) domain of the nsp12 protein-coding sequence as it was distant from the specimens considered by the International Committee on Taxonomy of Viruses (ICTV). Finally, by performing High-Throughput Sequencing, we were able to find contigs mostly belonging to domains of the replicase of bat coronaviruses related to American bats of the Molossidae and Vespertilionidae families.

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巴西蝙蝠（Molossus sp.）中一种冠状病毒的检测。

由于COVID-19大流行及其起源方面的不确定性，近年来人们对研究野生动物中的冠状病毒越来越感兴趣。蝙蝠是迄今为止冠状病毒最多样化的宿主，包括在人类中引起疫情的病毒的祖先。尽管在巴西，关于蝙蝠中冠状病毒的信息已经扩大，但它们仍然不具有代表性。为了帮助阐明这个问题，我们从巴西的两个州收集了175个不同种类的蝙蝠样本。在这里，我们报告了一种以前未知的存在于一只蝙蝠（Molossus sp.）的冠状病毒。系统发育分析显示，该病毒与巴西和阿根廷的甲型冠状病毒关系密切，但由于与国际病毒分类委员会（ICTV）考虑的标本距离较远，因此无法利用nsp12蛋白编码序列的rna依赖rna聚合酶（RdRp）结构域确定该病毒的亚属或种。最后，通过进行高通量测序，我们能够找到大部分属于蝙蝠冠状病毒复制酶的结构域，这些结构域与Molossidae和Vespertilionidae科的美洲蝙蝠有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Evolution 生物-进化生物学

CiteScore

5.50

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： Journal of Molecular Evolution covers experimental, computational, and theoretical work aimed at deciphering features of molecular evolution and the processes bearing on these features, from the initial formation of macromolecular systems through their evolution at the molecular level, the co-evolution of their functions in cellular and organismal systems, and their influence on organismal adaptation, speciation, and ecology. Topics addressed include the evolution of informational macromolecules and their relation to more complex levels of biological organization, including populations and taxa, as well as the molecular basis for the evolution of ecological interactions of species and the use of molecular data to infer fundamental processes in evolutionary ecology. This coverage accommodates such subfields as new genome sequences, comparative structural and functional genomics, population genetics, the molecular evolution of development, the evolution of gene regulation and gene interaction networks, and in vitro evolution of DNA and RNA, molecular evolutionary ecology, and the development of methods and theory that enable molecular evolutionary inference, including but not limited to, phylogenetic methods.